OsRAN2, essential for mitosis, enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress
OsRAN2, essential for mitosis, enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress
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DOI：
10.1111/j.1365-3040.2010.02225.x
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发表时间：
2011-01-01
影响因子：
7.3
通讯作者：
Chong, Kang
中科院分区：
文献类型：
--
作者：
Chen, Na;Xu, Yunyuan;Chong, Kang
随着全球气候变化，异常低温影响了世界大米的产量。许多基因已被证明对于水稻冷耐受性状的分子改善至关重要。但是，对它们对冷应激的反应的分子细胞机制知之甚少。在这里，我们调查了大米冷应激期间参与细胞分裂调节的OSRAN2。在冷处理下，Osran2的表达增加，但在盐和干旱胁迫期间没有增加。平均根有丝分裂指数与OSRAN2的表达水平密切相关。敲低的转基因水稻线在有丝分裂过程中表现出异常的纺锤体组织，并在发育过程中发育不良。 OSRAN2的过表达增强了大米的冷耐受性。过表达OSRAN2的转基因水稻显示细胞分裂，核内小管蛋白的细胞比例降低，并在冷条件下形成正常的核膜。我们的研究表明，通过促进有丝分裂结束时促进核内微管蛋白的正常出口来维持细胞分裂，通过维持细胞分裂来调节水稻的冷耐药机制。这种见解可以帮助改善大米的冷耐受性特征。
With global climate change, abnormally low temperatures have affected the world's rice production. Many genes have been shown to be essential for molecular improvement of rice cold-tolerance traits. However, less is known about the molecular cellular mechanism of their response to cold stress. Here, we investigated OsRAN2 involved in regulation of cell division during cold stress in rice. Expression of OsRAN2 was increased under cold treatment, but not during salt and drought stress. The mean root mitotic index was closely related to the expression level of OsRAN2. Knockdown transgenic rice lines showed an aberrant organization of spindles during mitosis and stunted growth during development. Overexpression of OsRAN2 enhanced cold tolerance in rice. The transgenic rice overexpressing OsRAN2 showed maintained cell division, decreased proportion of cells with intranuclear tubulin and formation of a normal nuclear envelope under the cold condition. Our study suggests a mechanism for OsRAN2 in regulating cold resistance in rice by maintaining cell division through promoting the normal export of intranuclear tubulin at the end of mitosis. This insight could help improve the cold-tolerance trait in rice.