Biosynthesis of silver nanoparticles from Schizophyllum radiatum HE 863742.1: their characterization and antimicrobial activity.
Biosynthesis of silver nanoparticles from Schizophyllum radiatum HE 863742.1: their characterization and antimicrobial activity.
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DOI：
10.1007/s13205-013-0138-0
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发表时间：
2014-06
期刊：
影响因子：
2.8
通讯作者：
Singara Charya MA
中科院分区：
文献类型：
--
作者：
Metuku RP;Pabba S;Burra S;Hima Bindu N SV;Gudikandula K;Singara Charya MA
开发可靠且环保的银纳米颗粒合成工艺是纳米技术应用领域的重要一步。实现这一目标的选择之一是使用自然生物过程。它们比涉及与环境毒性相关的化学试剂的常规方法具有优势。本研究证明了使用白色腐烂真菌（具有GenBank登录号HE 863742.1的辐射裂褶菌）的稳定银纳米颗粒的细胞外合成。分离细胞后获得的种子培养基的上清液已用于合成银纳米颗粒。采用FT-IR、XRD对合成的银纳米粒子的形貌和结构进行了表征，紫外-可见光谱在420-430 nm范围内显示出银纳米粒子的等离子体吸收峰。扫描电子显微镜显微照片显示形成了在10-40 nm范围内分散良好的银纳米颗粒。研究了不同碳源、成粒时间对纳米粒的影响，并与银溶液和标准链霉素进行了比较。还原过程是细胞外的且快速的，这可能导致开发用于合成银纳米颗粒的简单生物过程。
Development of reliable and eco-friendly process for synthesis of silver nanoparticles is an important step in the field of application in nanotechnology. One of the options to achieve this objective is to use natural biological processes. They have an advantage over conventional methods involving chemical agents associated with environmental toxicity. This study demonstrates the extra-cellular synthesis of stable silver nanoparticles using the white rot fungus, Schizophyllum radiatum with GenBank Accession no HE 863742.1. The supernatant of the seed media obtained after separating the cells has been used for the synthesis of silver nanoparticles. The morphology and structure of synthesized silver nanoparticles were characterized using FT-IR, XRD, UV–visible spectrum of the aqueous medium containing silver ion showed a peak in the range of 420–430 nm corresponding to the Plasmon absorbance of silver nanoparticles. Scanning electron microscopy micrograph showed formation of well-dispersed silver nanoparticles in the range of 10–40 nm. The effect of different carbon sources and the time taken for formation particles and the anti-microbial activity of synthesized nanoparticles were carried and compared with silver nitrate solution and with standard streptomycin. The process of reduction being extra-cellular and fast may lead to the development of an easy bioprocess for synthesis of silver nanoparticles.
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