Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels
Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels
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DOI：
10.1155/2015/434876
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Venkatraman, Subbu S.
中科院分区：
文献类型：
--
作者：
Agrawal, Animesh;Lee, Bae Hoon;Venkatraman, Subbu S.
已经开发了一种方法来诱导和保持血管平滑肌细胞的收缩表型，作为开发具有最小顺应性失配的仿生血管构建体的第一步。将熔纺的PCL纤维沉积在心轴上以形成直径为10 μ m的排列纤维。通过在壳聚糖溶液中浸涂，纤维被粘合成定向排列。这就形成了一个10 μ m深× 10 μ m宽的平行凹槽表面，呈现出一个壳聚糖表面层，以促进细胞表面相互作用。对齐的纤维表面用于培养存在于血管壁中的细胞，特别是成纤维细胞和平滑肌细胞。这种形貌诱导细胞取向的“表面引导”，其采用细长的纺锤状形态，而未图案化的对照表面上的细胞没有显示出这种取向，呈现更多的菱形形状。通过培养数天后α-SMA表达的保留证明了对齐支架上VSMC收缩表型的保留。在由聚乳酸己内酯制成的原型血管移植物假体上评估该效应; VSMC沿无纤维管纵向沿着排列，而对于排列的纤维涂层管，VSMC在所需的周向方向上排列。
A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 mu m in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 mu m deep by 10 mu m across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced "surface guidance" over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of alpha-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.