TISSUE PO2 OF ORTHOTOPIC 9L AND C6 GLIOMAS AND TUMOR-SPECIFIC RESPONSE TO RADIOTHERAPY AND HYPEROXYGENATION
TISSUE PO2 OF ORTHOTOPIC 9L AND C6 GLIOMAS AND TUMOR-SPECIFIC RESPONSE TO RADIOTHERAPY AND HYPEROXYGENATION
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DOI：
10.1016/j.ijrobp.2008.10.025
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发表时间：
2009-03-01
影响因子：
7
通讯作者：
Swartz, Harold M.
中科院分区：
文献类型：
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作者：
Khan, Nadeem;Lin, Hongbin;Swartz, Harold M.
目的：肿瘤缺氧是一个众所周知的治疗问题；然而，缺乏重复测量神经胶质瘤氧分压（pO（2））的方法限制了优化治疗方法的能力。我们使用一种新的且可能广泛使用的方法，即多部位体内电子顺磁共振血氧测定法，报告了 9.3 Gy 辐射和卡波金吸入对大鼠原位 9L 和 C6 神经胶质瘤以及对侧大脑 pO(2) 的影响。方法和材料：在同种大鼠的左半球建立脑内 9L 和 C6 肿瘤，电子顺磁共振血氧测定法成功用于重复组织9.3 Gy 辐射后和连续 5 天卡波金呼吸期间的 pO(2) 测量。结果：脑内 9L 神经胶质瘤的 pO(2) 为 30-32 mm Hg，C6 神经胶质瘤相对缺氧，pO(2) 为 12-14 mm Hg (p < 0.05)。患有 9L 或 C6 神经胶质瘤的大鼠对侧大脑的组织 pO(2) 为 40-45 mm Hg。辐射仅导致 9L 神经胶质瘤的 pO(2) 显着增加。在吸入卡波金的大鼠中观察到 9L 和 C6 神经胶质瘤的 pO(2) 显着增加，但在 9L 神经胶质瘤中进行 5 天的重复实验期间，这种效应下降。结论：这些结果突出了辐射 (9.3.Gy) 对组织 pO(2) 的肿瘤特异性影响以及对卡波金吸入的不同反应。电子顺磁共振血氧测定法提供组织 pO(2) 的直接重复测量的能力对于理解治疗期间缺氧的动态具有至关重要的作用，然后可以通过在改善肿瘤氧合的时间安排剂量来优化治疗。 (C) 2009 爱思唯尔公司。
Purpose: Tumor hypoxia is a well-known therapeutic problem; however, a lack of methods for repeated measurements of glioma partial pressure of oxygen (pO(2)) limits the ability to optimize the therapeutic approaches. We report the effects of 9.3 Gy of radiation and carbogen inhalation on orthotopic 9L and C6 gliomas and on the contralateral brain pO(2) in rats using a new and potentially widely useful method, multisite in vivo electron paramagnetic resonance oximetry.Methods and Materials: Intracerebral 9L and C6 tumors were established in the left hemisphere of syngeneic rats, and electron paramagnetic resonance oximetry was successfully used for repeated tissue pO(2) measurements after 9.3 Gy of radiation and during carbogen breathing for 5 consecutive days.Results: Intracerebral 9L gliomas had a pO(2) of 30-32 mm Hg and C6 gliomas were relatively hypoxic, with a pO(2) of 12-14 mm Hg (p < 0.05). The tissue pO(2) of the contralateral brain was 40-45 mm Hg in rats with either 9L or C6 gliomas. Irradiation resulted in a significant increase in pO(2) of the 9L gliomas only. A significant increase in the pO(2) of the 9L and C6 gliomas was observed in rats breathing carbogen, but this effect decreased during 5 days of repeated experiments in the 9L gliomas.Conclusion: These results highlight the tumor-specific effect of radiation (9.3.Gy) on tissue pO(2) and the different responses to carbogen inhalation. The ability of electron paramagnetic resonance oximetry to provide direct repeated measurements of tissue pO(2) could have a vital role in understanding the dynamics of hypoxia during therapy that could then he optimized by scheduling doses at times of improved tumor oxygenation. (C) 2009 Elsevier Inc.