Identification of 3-Hydroxykynurenine and Xanthurenic Acid and Quantitation of 3-Hydroxykynurenine Transaminase Activity Using HPLC with Electrochemical Detection
Identification of 3-Hydroxykynurenine and Xanthurenic Acid and Quantitation of 3-Hydroxykynurenine Transaminase Activity Using HPLC with Electrochemical Detection
复制标题
使用 HPLC 和电化学检测技术鉴定 3-羟基犬尿氨酸和黄尿酸并定量 3-羟基犬尿氨酸转氨酶活性
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Guoyu Li
中科院分区：
文献类型：
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作者：
Jianyong Li;Guoyu Li
摘要以蚊幼虫为试材，采用高效液相色谱-电化学检测法（HPLC-ED）对3-羟基犬尿氨酸和黄尿酸进行了鉴定，并测定了3-羟基犬尿氨酸转氨酶的活性。结果表明，3-羟基犬尿氨酸和黄尿酸粗生物样品中，可以鉴定基于其电化学和色谱行为在HPLC-ED分析。黄尿酸是通过3-羟基犬尿氨酸的转氨基作用产生的，并且蚊子幼虫提取物中黄尿酸的鉴定也导致检测该昆虫物种中的3-羟基犬尿氨酸转氨酶活性。同时，以蚊幼虫蛋白为酶源，建立了检测和定量3-羟基犬尿氨酸转氨酶活性的方法，并以小鼠肝、肾匀浆上清液为酶源，验证了该方法对其他来源样品的适用性。
Abstract Identification of 3-hydroxykynurenine and xanthurenic acid and determination of 3-hydroxykynurenine transaminase activity using HPLC with electrochemical detection (HPLC-ED) are described using mosquito larval samples as testing materials. Results indicate that 3-hydroxykynurenine and xanthurenic acid in crude biological samples can be identified based on their electrochemical and chromatographic behaviors during HPLC-ED analysis. Xanthurenic acid is produced through transamination of 3-hydroxykynurenine, and identification of xanthurenic acid in the mosquito larval extract also leads to the detection of 3-hydroxykynurenine transaminase activity in this insect species. Using the same HPLC-ED technique, a method to detect and quantitate 3-hydroxykynurenine transaminase activity is developed using isolated mosquito larval protein as enzyme source, and the applicability of the HPLC-ED transaminase assay for samples from other resources is demonstrated using supernatant of mouse liver and kidney homo...