Using light to establish and measure stiffness gradients in three-dimensional engineered tissues
Using light to establish and measure stiffness gradients in three-dimensional engineered tissues
复制标题
使用光建立和测量三维工程组织中的刚度梯度
DOI：
10.1117/12.2595590
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Castillo, U
中科院分区：
文献类型：
--
作者：
Jagiello, A;Castillo, U
纤维系统如胶原蛋白或纤维蛋白内的细胞-细胞外基质（ECM）相互作用的研究具有挑战性，特别是如果不能监测细胞周围的硬度。在这里，我们提出了我们的基于光的方法，结合多轴光镊主动微流变学的分子交联的非侵入性图案化，以映射ECM刚度景观。该方法允许我们生成规定的刚度梯度和相关的各向异性，其对天然细胞周围ECM的刚度进行建模。图案化交联诱导应变硬化和测量的刚度梯度与预测的应变场一致。迁移细胞对这些梯度的反应通过F-肌动蛋白分布和形态学特性的变化来评估。
Studies of cell-extracellular matrix (ECM) interactions within fibrous systems such as collagen or fibrin are challenging, particularly if peri-cellular stiffness cannot be monitored. Here we present our light-based method for non-invasive patterning of molecular crosslinking combined with multi-axes optical tweezers active microrheology to map ECM stiffness landscapes. This method allows us to generate prescribed stiffness gradients and associated anisotropies, which model stiffness of the natural peri-cellular ECM. Patterned crosslinking induces strain hardening and measured stiffness gradients are in agreement with predicted strain fields. Migratory cells respond to these gradients as assessed by change in F-actin distribution and morphological properties.