Micropatterning different cell types with microarray amplification of natural directional persistence.
Micropatterning different cell types with microarray amplification of natural directional persistence.
复制标题
通过自然定向持久性的微阵列放大对不同细胞类型进行微图案化。
DOI：
10.1002/adhm.201200141
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发表时间：
2013
影响因子：
10
通讯作者：
Ho,Chia-Chi
中科院分区：
文献类型：
--
作者：
Mun,Kyu-Shik;Kumar,Girish;Co,CarlosC;Ho,Chia-Chi
在体内，不同的细胞类型以特定的模式组装形成功能组织。通过设计支架材料在正确的时间将细胞精确地引导到正确的位置，在体外重现这一过程对于下一代生物材料非常重要。在这里，使用自然定向持久性的微阵列扩增（MANDIP），通过指导成纤维细胞和内皮细胞的长距离迁移来证明它们的同时组装。定向持久性的放大通过形态诱导的极性和单个微尺寸粘附岛的不对称定位发生，所述粘附岛限制板状伪足附着，从而限制迁移到一个预设方向。不同的MANDIP设计上的细胞迁移的定量分析产生洞察的非对称岛的形状和它们的安排的相对重要性。该方法能够在大面积上以微米级精度对不同细胞类型进行空间图案化，以研究复杂组织结构中的细胞-细胞相互作用。
In vivo, different cell types assemble in specific patterns to form functional tissues. Reproducing this process in vitro by designing scaffold materials to direct cells precisely to the right locations at the right time is important for the next generation of biomaterials. Here, using microarray amplification of natural directional persistence (MANDIP), simultaneous assembly of fibroblasts and endothelial cells is demonstrated by directing their long‐range migration. Amplification of the directional persistence occurs through morphology‐induced polarity and the asymmetric positioning of individual microsized adhesive islands that restrict lamellipodia attachment, and thus migration, to one preset direction. Quantitative analysis of cell migration on different MANDIP designs yields insight to the relative importance of the asymmetric island shapes and their arrangement. The approach enables spatial patterning of different cell types with micrometer‐scale precision over large areas for investigation of cell‐cell interactions within complex tissue architectures.