Characterization of gut-associated lymphoid tissue (GALT) of normal rhesus macaques
Characterization of gut-associated lymphoid tissue (GALT) of normal rhesus macaques
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DOI：
10.1006/clin.1996.4318
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发表时间：
1997-03-01
期刊：
影响因子：
--
通讯作者：
Lackner, AA
中科院分区：
文献类型：
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作者：
Veazey, RS;Rosenzweig, M;Lackner, AA
本研究描述了正常健康恒河猴肠道相关淋巴组织（GALT）的特征，并将T和B细胞亚群的百分比与全身淋巴组织的百分比进行了比较。对成年和幼年正常恒河猴的全身淋巴组织（脾脏、腋窝和腹股沟淋巴结）、肠系膜淋巴结（MLN）以及空肠、回肠和结肠的肠上皮（IEL）和固有层（LPL）的淋巴细胞进行了检查。通过双色或三色流式细胞术分析淋巴细胞的CD 2、CD 3、CD 4、CD 8、CD 25、γ δ TCR和CD 20表达。通过免疫组织化学检查空肠、回肠和结肠切片的CD 3、CD 20和CD 103表达。还通过免疫组织化学检查派伊尔集合淋巴结的CD 3、CD 4、CDS和CD 20表达。IEL和LPL以CD 103（+）、CD 3（+）T细胞为主，CD 20（+）B细胞较少。IEL主要为CD 3（+）CD 8（+）（63-80%），CD 4（+）细胞极少，而LPL中的CD 4：CD 8比值范围为0.74 - 1.3。3 - 38%的IEL为γ δ TCR阳性，但γ δ表达在LPL和MLN中罕见，γ δ TCR表达在年轻动物的IEL中也较高。与IEL和全身组织相比，LPL具有更高的CD 25表达，特别是在老年动物中。GALT中还观察到CD 4（+）CD 8（+）双阳性和CD 3（+）CD 4（-）CD 8（-）双阴性细胞。这些结果表明恒河猴的GALT与人的GALT非常相似，进一步证明了这些动物作为各种肠道疾病模型的合理性。(C)北京：科学出版社.
This study characterizes the gut-associated lymphoid tissue (GALT)of normal healthy rhesus macaques and compares the percentages of T and B cell subsets to those of systemic lymphoid tissue. Lymphocytes from the systemic lymphoid tissue (spleen, axillary, and inguinal lymph nodes), mesenteric lymph nodes (MLN), and intestinal epithelium (IEL) and lamina propria (LPL) of the jejunum, ileum, and colon were examined from both adult and juvenile, normal rhesus macaques. Lymphocytes were analyzed for expression of CD2, CD3, CD4, CD8, CD25, gamma delta TCR, and CD20 by two- or three-color flow cytometric analysis, Sections of jejunum, ileum, and colon were examined for CD3, CD20, and CD103 expression by immunohistochemistry. Peyer's patches were also examined for CD3, CD4, CDS, and CD20 expression by immunohistochemistry. Most IEL and LPL were CD103(+), CD3(+) T cells with significantly fewer CD20(+) B cells. The IEL were predominantly CD3(+)CD8(+) (63-80%), with very few CD4(+) cells, whereas CD4:CD8 ratios in the LPL ranged from 0.74 to 1.3. Three to 38% of the IEL were gamma delta TCR positive, but gamma delta expression was rare in the LPL and MLN, gamma delta TCR expression was also higher in the IEL of younger animals. LPL had higher expression of CD25 compared to IEL and systemic tissues, particularly in aged animals. CD4(+)CD8(+), double-positive and CD3(+)CD4(-)CD8(-) double-negative cells were also observed in GALT, These results demonstrate that GALT of rhesus macaques is remarkably similar to that of humans, further justifying the use of these animals as models for various intestinal disorders. (C) 1997 Academic Press.