Larvicidal Activity of Mosquito Sterol Carrier Protein-2 Inhibitors to the Insecticide-Resistant Mosquito Culex quinquefasciatus (Diptera: Culicidae)
Larvicidal Activity of Mosquito Sterol Carrier Protein-2 Inhibitors to the Insecticide-Resistant Mosquito Culex quinquefasciatus (Diptera: Culicidae)
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DOI：
10.1603/033.046.0626
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发表时间：
2009-11-01
影响因子：
2.1
通讯作者：
Liu, Nannan
中科院分区：
文献类型：
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作者：
Li, Ting;Lan, Que;Liu, Nannan
AeSCP-2抑制剂（SCPI）与胆固醇竞争结合蚊子固醇载体蛋白-2（AeSCP-2），已知该蛋白有助于蚊子细胞摄取胆固醇。因此，AeSCP-2抑制剂-1（SCPI-1）和抑制剂-2（SCPI-2）对致倦库蚊（双翅目：库蚊科）的杀幼虫活性在对杀虫剂具有抗性的库蚊HAmCq（G9）、MAmCq（G2）和BAmCq（G 0）中进行了检测。与敏感的S-Lab品系相比，所有抗性库蚊品系对SCPI-1和SCPI-2抑制剂表现出相似的敏感性。当AeSCP-2抑制剂与氯菊酯同时施用时，氯菊酯对所有四种库蚊品系的二龄幼虫的毒性增加，表明AeSCP-2抑制剂对氯菊酯对库蚊的毒性具有协同效应。SCPI-1和SCPI-2抑制剂均导致高抗性菌株HAmCq（G9）中氯菊酯抗性水平降低2.4至3倍。这一结果表明，AeSCP-2抑制剂的作用模式，即通过抑制蚊子细胞中AeSCP-2的功能来减少胆固醇的摄取，可能会干扰HAmCq（G9）蚊子品系中控制氯菊酯抗性的机制或能力。
AeSCP-2 inhibitors (SCPIs) compete with cholesterol for binding to a mosquito sterol carrier protein-2 (AeSCP-2) known to aid in the uptake of cholesterol in mosquito cells. The larvicidal activities of AeSCP-2 inhibitor-1 (SCPI-1) and inhibitor-2 (SCPI-2) against Culex quinquefasciatus Say (Diptera: Culicidae) were therefore examined in insecticide-resistant Culex mosquitoes HAmCq(G9), MAmCq(G2), and BAmCq(G0). All of the resistant Culex mosquito strains exhibited similar sensitivity to SCPI-1 and SCPI-2 inhibitors compared with a susceptible S-Lab strain. When an AeSCP-2 inhibitor was applied simultaneously with permethrin, the toxicity of permethrin to the second-instar larvae of all four strains of Culex mosquitoes increased, suggesting a synergistic effect of AeSCP-2 inhibitors on the toxicity of permethrin against Culex mosquitoes. Both SCPI-1 and SCPI-2 inhibitors caused a 2.4- to 3-fold reduction in the level of permethrin resistance in the highly resistant strain HAmCq(G9). This result suggests that the mode of action of the AeSCP-2 inhibitors, which reduces the uptake of cholesterol by inhibiting the function of AeSCP-2 in mosquito cells, may interfere with the mechanisms or ability that govern permethrin resistance in the HAmCq(G9) mosquito strain.