Synergistic regulatory effects of microRNAs on brain glioma cells.
Synergistic regulatory effects of microRNAs on brain glioma cells.
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DOI：
10.3892/mmr.2017.6709
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发表时间：
2017-08
影响因子：
3.4
通讯作者：
Yilei Zhao;Xiaomeng Cui;Wenliang Zhu;Xin Chen;Chen Shen;Zhendong Liu;Guang Yang;Yaohua Liu;Shiguang Zhao
中科院分区：
文献类型：
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作者：
Yilei Zhao;Xiaomeng Cui;Wenliang Zhu;Xin Chen;Chen Shen;Zhendong Liu;Guang Yang;Yaohua Liu;Shiguang Zhao
神经胶质瘤是中枢神经系统最常见的癌症类型之一，并且难以治愈。由于缺乏针对神经胶质瘤的特异性治疗，神经胶质瘤患者的死亡率很高。 MicroRNA (miRNA) 参与神经胶质瘤的发病机制，特定 miRNA 的上调可促进细胞增殖，而诱导细胞凋亡的 miRNA 在神经胶质瘤中显着下调。因此，miRNA可能是神经胶质瘤发病机制的重要贡献者。在本研究中，以 miRNA-miRNA 对的形式对 9 个 miRNA 进行了研究，测量的细胞活力与协同作用预测的结果一致。 U87细胞中上调的miRNA之间发生了广泛的协同作用，而下调的miRNA则很少表现出协同作用。使用表现出强协同作用的 miRNA-miRNA 对进行治疗，增加了这些 miRNA 对肿瘤细胞的抑制作用，并且与每个 miRNA 单独抑制作用的总和相比，组合抑制作用有所增加。本研究使用细胞活力测定、TUNEL 染色和流式细胞术证明，共转染 miR-20a 和 miR-21 抑制剂对促进 U87 细胞凋亡具有最高的协同效应。本研究的结果为 miRNA 在神经胶质瘤治疗中的潜在用途提供了重要的见解。
Glioma is among the most common types of cancer of the central nervous system and is difficult to cure. Due to the lack of glioma-specific treatments, patients with glioma exhibit high mortality rates. MicroRNAs (miRNAs) participate in the pathogenesis of glioma, and upregulation of specific miRNAs promotes cell proliferation, whereas apoptosis‑inducing miRNAs are markedly downregulated in the context of glioma. Therefore, miRNAs may be important contributors to the pathogenesis of glioma. In the present study, nine miRNAs were investigated as miRNA‑miRNA pairs, and the measured cell viabilities were consistent with the results of synergy predictions. Extensive synergy occurred among upregulated miRNAs in U87 cells, whereas downregulated miRNAs rarely exhibited synergism. Treatment with an miRNA‑miRNA pair exhibiting strong synergy increased the inhibitory effects of these miRNAs on tumor cells, and the combined inhibitory effects were increased compared with the sum of the individual inhibitory effects of each miRNA. Using cell viability assays, TUNEL staining, and flow cytometry, the present study demonstrates that cotransfection with miR‑20a and miR‑21inhibitors resulted in the highest synergistic effect on the promotion of apoptosis in U87 cells. The results of the present study provide important insights into the potential use of miRNAs in the treatment of glioma.