Near Full-Length Sequence Analysis of a Novel HIV-1 C/CRF01_AE Recombinant Variant from Yunnan, China
Near Full-Length Sequence Analysis of a Novel HIV-1 C/CRF01_AE Recombinant Variant from Yunnan, China
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来自中国云南的新型 HIV-1 C/CRF01_AE 重组变体的近全长序列分析
DOI：
10.1089/aid.2010.0116
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发表时间：
2010-11-01
影响因子：
1.5
通讯作者：
Li, Jingyun
中科院分区：
文献类型：
--
作者：
Li, Lin;Chen, Lili;Li, Jingyun
重组极大地增加了HIV-1的总体遗传复杂性，特别是在多种亚型循环的人群中。云南是中国地区艾滋病疫情最严重的省份，流行着多种亚型和CRF病毒。在这项研究中，我们报道了一种新的HIV-1重组，涉及C和CRF01_AE。将接近全长的基因组逆转录并扩增成两半，其中有1kb的重叠区。扩增产物经纯化后直接测序。序列分析表明，该基因组大部分为CRF01_AE，在GAG和POL区域沿基因组插入了3个C亚型片段。这是云南地区首次发现C亚型与CRF01AE型重组的新基因中国。需要更多的工作来检查新重组的流行病学意义。
Recombination contributes substantially to the overall genetic complexity of HIV-1, especially in populations in which multiple subtypes circulate. Yunnan is a province in China with most severely HIV epidemic where many subtypes and CRFs of HIV-1 are circulating. In this study, we report a novel HIV-1 recombination involving C and CRF01_AE. The near full-length genome was reverse transcripted and amplified in two halves with the 1 kb overlap regions. The PCR products were sequenced directly after purification. Sequence analysis showed that the majority of the genome was CRF01_AE, three fragments of C subtype were inserted along the genome in gag and pol region. This is the first report of a novel recombination involving subtype C and CRF01_AE in Yunnan, China. More work is needed to check the epidemiologic significance of the new recombination.