Fluorochrome in monitoring atmospheric bioaerosols and correlations with meteoro ogical factors and air pollutants
Fluorochrome in monitoring atmospheric bioaerosols and correlations with meteoro ogical factors and air pollutants
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DOI：
10.1080/02786820701383181
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发表时间：
2007-07-01
影响因子：
5.2
通讯作者：
Li, Chih-Shan
中科院分区：
文献类型：
--
作者：
Chi, Miao-Ching;Li, Chih-Shan
用5种荧光染料(AO、DAPI、SYTO-13、PI和YOPRO-1)用荧光荧光显微镜(EFM/FL)监测了环境大气中生物气溶胶的特征。同时对生物气溶胶与气象因子和空气污染物的相关性进行了评估。然后将EFM/FL的结果与常用的基于培养的方法进行比较。非培养法测得的微生物细胞总数平均约为8×10(-5)cell/m(3)。然而，基于培养的方法将生物气溶胶浓度低估了100到1000倍。PI染色EFM/FL法平均存活率为0.66，YOPRO-1染色平均存活率为0.35，培养法平均存活率为0.0012。EFM/FL培养的成活率远高于可培养能力。总之，以培养为基础的方法严重低估了微生物细胞的总浓度和活性。此外，基于基于培养和非基于培养的方法的结果，总生物气溶胶浓度可能与温度、降雨量和紫外线影响的结果强烈相关。然而，生物气溶胶浓度与空气污染物之间的相关性很弱。综上所述，利用EFM/FL和基于培养的方法成功地对大气生物气溶胶的微生物细胞总数和活性进行了比对。此外，我们的研究表明，在大气样本中，通过非培养方法，生物气溶胶与气象因素之间存在很强的相关性。
The characteristics of bioaerosols in the ambient atmosphere were monitored by using epifluorescence microscopy with fluorochrome (EFM/FL) with five fluorescent dyes (AO, DAPI, SYTO-13, PI, and YOPRO-1). The correlations of bioaerosols with meteorological factors and air pollutants were simultaneously assessed. Results from EFM/FL were then compared with those using a commonly used culture-based method. The total microbial cell concentrations measured by the non-culture-based methods averaged about 8 x 10(-5) Cells/m(3). However, culture-based method underestimated bioaerosol concentrations by the factor of 100 to 1000. The average viabilities were 0.66 by EFM/FL with PI staining, 0.35 with YOPRO-1 staining, and 0.0012 by the culture-based method. The viability by EFM/FL was much higher than the culturability. In summary, the total microbial cell concentration and viability were highly underestimated by the culture-based method. Moreover, based on culture-based and non-culture-based methods results, the total bioaerosol concentrations could be strongly correlated with a result of the temperature, rainfall, and UV light influence. However, there were weak correlations between bioaerosol concentrations and air pollutants. In conclusion, the intercomparisons of the total microbial cell concentration and viability of atmospheric bioaerosols were successfully assessed by EFM/FL and culture-based method. In addition, our study demonstrated that strong correlations between bioaerosols and meteorological factors by non-culture-based methods in atmospheric samples.