A 4D single-cell protein atlas of transcription factors delineates spatiotemporal patterning during embryogenesis
A 4D single-cell protein atlas of transcription factors delineates spatiotemporal patterning during embryogenesis
复制标题
转录因子的 4D 单细胞蛋白质图谱描绘了胚胎发生过程中的时空模式
DOI：
10.1038/s41592-021-01216-1
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发表时间：
2021-07-26
期刊：
影响因子：
48
通讯作者：
Du, Zhuo
中科院分区：
文献类型：
--
作者：
Ma, Xuehua;Zhao, Zhiguang;Du, Zhuo
复杂的生物过程，如胚胎发生，需要在空间和时间上精确协调细胞分化程序。利用蛋白质融合荧光报告和四维实时成像，我们提出了一个蛋白质表达图谱的转录因子（TF）映射到发育细胞系在Caelinghabditis elegansembryogenesis，在单细胞分辨率。该图谱揭示了TF表达的时空组合代码，以及指定发育状态的谱系特异性、组织特异性和时间特异性TF的级联。该图集揭示了胚胎发生的调节因子，包括皮肤特异性因子在神经发生中的意想不到的作用和未表征的TF在会聚肌分化中的关键功能。在系统水平上，该图谱提供了一个机会，以模拟细胞状态-命运的关系，揭示了功能相关的细胞内的谱系依赖的状态多样性和发展状态进展的曲折轨迹。总的来说，这个单细胞蛋白质图谱代表了一个有价值的资源，阐明后生动物胚胎发生的分子和系统水平。
Complex biological processes such as embryogenesis require precise coordination of cell differentiation programs across both space and time. Using protein-fusion fluorescent reporters and four-dimensional live imaging, we present a protein expression atlas of transcription factors (TFs) mapped onto developmental cell lineages duringCaenorhabditis elegansembryogenesis, at single-cell resolution. This atlas reveals a spatiotemporal combinatorial code of TF expression, and a cascade of lineage-specific, tissue-specific and time-specific TFs that specify developmental states. The atlas uncovers regulators of embryogenesis, including an unexpected role of a skin specifier in neurogenesis and the critical function of an uncharacterized TF in convergent muscle differentiation. At the systems level, the atlas provides an opportunity to model cell state–fate relationships, revealing a lineage-dependent state diversity within functionally related cells and a winding trajectory of developmental state progression. Collectively, this single-cell protein atlas represents a valuable resource for elucidating metazoan embryogenesis at the molecular and systems levels.