Comparative studies on the protein profiles and hydrophobicity of strains of Streptococcus mutans serotype c.
Comparative studies on the protein profiles and hydrophobicity of strains of Streptococcus mutans serotype c.
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变形链球菌c血清型菌株蛋白质谱和疏水性的比较研究。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
A. J. Wicken
中科院分区：
文献类型：
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作者：
K. Knox;L. Hardy;A. J. Wicken
12株变形链球菌血清型c生长在分批培养与葡萄糖在恒定的pH值（6.0）和一些性能进行了比较。根据它们的细胞和胞外蛋白质谱，菌株被分为三组，I，II和III，分别含有5，4和3株。如果生物体在pH 6.0下用果糖代替葡萄糖或用葡萄糖但没有pH控制生长，则特定菌株的胞外蛋白质谱不同。在pH 6.0的含葡萄糖培养基中生长的第III组菌株产生的胞外蛋白的总量是第I组和第II组菌株产生的胞外蛋白的总量的几倍，第I组和第II组菌株也更疏水。潜在重要的蛋白质之一是P1，也称为抗原B或I/II，并且显示其完全存在于组III菌株的培养液中，但主要与来自组I和II菌株的细胞相关。大约一半的P1细胞相关部分可以用热十二烷基硫酸钠去除。
Twelve strains of Streptococcus mutans serotype c were grown in batch culture with glucose at constant pH (6.0) and a number of properties compared. On the basis of their cellular and extracellular protein profiles, the strains were divided into three groups, I, II and III, containing five, four and three strains, respectively. The extracellular protein profiles for a particular strain differed if the organisms were grown either at pH 6.0 with fructose instead of glucose or with glucose but without pH control. The total amount of extracellular protein produced by group III strains grown in glucose-containing medium at pH 6.0 was several times that produced by strains of groups I and II, which were also more hydrophobic. One of the potentially important proteins is P1, also called antigen B or I/II, and it was shown to be entirely in the culture fluid of group III strains but mostly cell-associated from strains of groups I and II. Approximately half of the cell-associated fraction of P1 could be removed with hot sodium dodecyl sulphate.