Molecular authentication of Panax species
Molecular authentication of Panax species
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DOI：
10.1016/s0031-9422(98)00606-2
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发表时间：
1999-03-01
期刊：
影响因子：
3.8
通讯作者：
Wang, J
中科院分区：
文献类型：
--
作者：
Ngan, F;Shaw, P;Wang, J
以保守的植物序列为引物，扩增并测定了人参6种植物核糖体ITS1-5.8S-ITS2区的DNA序列。(东方人参)、西洋参(P.ququiefolius L.)(西洋参)、三七(P.notogiseng(Burkill)F.H.Chen(Sanchi))、西洋参P.Japan onicus C.A.Mey.(日本人参)、三叶人参(P.trifolius L.)和三叶人参(P.main Ting)，以及两种常见的人参混伪品紫茉莉(Mirabilis Jalapa L.)和商陆(Phytolacca acinosa Roxb.)基于该区域限制性片段长度多态性(RFLP)的鉴定方法能够区分人参和西洋参，并能区分人参和两种常见的有毒混伪品。更广泛地应用这种方法来鉴定其他形态相似的中药材是合理的。(C)1999爱思唯尔科学有限公司。保留所有权利。
Using conserved plant sequences as primers, the DNA sequences in the ribosomal ITS1-5.8S-ITS2 region have been amplified and determined for six Panax species, P. ginseng C. A. Mey. (Oriental ginseng), P. quinquefolius L. (American ginseng), P. notoginseng (Burkill) F. H. Chen (Sanchi), P. japonicus C. A. Mey. (Japanese ginseng), P. trifolius L. and P. major Ting, as well as two Common adulterants of ginseng, Mirabilis jalapa L. and Phytolacca acinosa Roxb. An authentication procedure based upon the restriction fragment length polymorphism (RFLP) in the region is able to differentiate between P, ginseng and P, quinquefolius, and to discriminate the ginsengs from the two common poisonous adulterants. Broader application of this approach to authenticate other morphologically similar Chinese medicinal materials is rationalised. (C) 1999 Elsevier Science Ltd. All rights reserved.