Isolation and characterization of bacterium producing lipid from short-chain fatty acids
Isolation and characterization of bacterium producing lipid from short-chain fatty acids
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DOI：
10.1016/j.biortech.2015.11.040
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发表时间：
2016-02-01
影响因子：
11.4
通讯作者：
Matsumoto, Mitsufumi
中科院分区：
文献类型：
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作者：
Okamura, Yoshiko;Nakai, Shota;Matsumoto, Mitsufumi
厌氧发酵会产生丙酸，丙酸会抑制微生物的生长，并在含有大量有机物的废水中积累。因此，我们分离出一株能够产生三酰甘油的丙酸同化细菌，用于废水处理。硝酸还原细菌。OM-1菌株可以在含有丙酸、醋酸、丁酸、戊酸和甘油的培养基中增殖，并在丙酸和醋酸或甘油同时存在的情况下产生三酰甘油。在含有醋酸、丙酸和甘油的复合模型废水中，该菌株表现出更高的转化率，适合于废水处理。此外，在含有醋酸-丙酸混合物的介质中，氮气消耗导致轻质油2-丁烯酸1-甲基乙酯的产生，但不产生三酰甘油。总体而言，我们的数据表明，OM-1菌株具有减少废水处理中活性污泥积累的潜力，并可能有助于生物柴油的生产。(C)2015爱思唯尔有限公司。保留所有权利。
Anaerobic fermentation generates propionic acid, which inhibits microbial growth and accumulates in wastewater containing increased amounts of organic matter. We therefore isolated a propionic acid-assimilating bacterium that could produce triacylglycerol, for use in wastewater treatment. Nitratireductor sp. strain OM-1 can proliferate in medium containing propionic, acetic, butyric, and valeric acids as well as glycerol, and produces triacylglycerol when both propionic and acetic acids or glycerol are present. In composite model wastewater containing acetic acid, propionic acid and glycerol, this strain shows an even higher conversion rate, suggesting that it is suitable for wastewater treatment. Further, nitrogen depletion in medium containing an acetic-propionic acid mixture resulted in the production of the light oil 2-butenoic acid 1-methylethyl ester, but not triacylglycerol. Collectively, our data indicate that strain OM-1 has the potential to reduce accumulation of activated sludge in wastewater treatment and may contribute to the production of biodiesel. (C) 2015 Elsevier Ltd. All rights reserved.