Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection
Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection
复制标题
DOI：
10.1016/s0168-1702(99)00073-8
复制
发表时间：
1999-10-01
期刊：
影响因子：
5
通讯作者：
Alonso, F
中科院分区：
文献类型：
--
作者：
Fuertes, LL;Doménech, N;Alonso, F
在体外研究了从实验感染中恢复的动物对猪繁殖与呼吸综合征（PRRS）病毒欧洲分离株的细胞免疫应答。这些猪的外周血单个核细胞（PBMC）在用PRRS病毒刺激时特异性增殖。这种反应直到接种后4周才检测到，并保持3个月以上。向培养物中加入阻断性单克隆抗体表明，这种增殖主要依赖于CD 4+细胞，并有SLA-II类分子的参与。通过用PRRS病毒刺激应答细胞建立的T细胞培养物，并在培养物中维持6周至3周，显示活化细胞内的CD 8 + CD 4+和CD 4-CD 8+亚群增加，根据其光散射参数进行门控，而CD 4 + CD 8-细胞沿着培养时间下降。在活化的细胞内，表达TcR γ δ受体的细胞也增加，其中大多数也对CD 8标记物呈阳性。通过RT-PCR，T细胞对病毒的反应显示出Th 1型细胞因子的产生模式。在培养期间，对K-562细胞的细胞毒活性从特异性裂解的15%增加到35%。这种细胞免疫应答可能在PRRS病毒的清除和感染的恢复中发挥相关作用。(C)1999 Elsevier Science B. V.保留所有权利。
The cellular immune response to a European isolate of porcine reproductive and respiratory syndrome (PRRS) virus in animals recovered from the experimental infection has been studied in vitro. Peripheral blood mononuclear cells (PBMC) from these pigs proliferated specifically when they were stimulated with PRRS virus. This response was not detectable until 4 weeks after inoculation and remained for more than 3 months. Addition of blocking monoclonal antibodies to the cultures showed that this proliferation was mainly dependent on CD4+ cells with the participation of SLA-class II molecules. T-cell cultures established by stimulating responding cells with PRRS virus and maintained in culture for lip to 3 weeks showed an increase of CD8+ CD4+ and CD4- CD8+ subsets within activated cells, gated according to their light scatter parameters, whereas CD4+ CD8- cells declined along the time in culture. Within the activated cells, those expressing the TcR gamma delta receptor also increased, being most of them also positive for the CD8 marker. By RT-PCR, T-cells responding to the virus showed a Th1 type cytokine production pattern. During the culture period the cytotoxic activity against K-562 cells increased from 15 to 35% of specific lysis. This cellular immune response may play a relevant role in the clearance of PRRS virus and the recovery of the infection. (C) 1999 Elsevier Science B.V. All rights reserved.