Ciliary neurotrophic factor in the olfactory bulb of rats and mice
Ciliary neurotrophic factor in the olfactory bulb of rats and mice
复制标题
DOI：
10.1016/s0306-4522(03)00211-2
复制
发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Carroll, P
中科院分区：
文献类型：
--
作者：
Asan, E;Langenhan, T;Carroll, P
睫状神经营养因子（CNTF）主要被认为是一种星形胶质细胞损伤因子，促进中枢神经系统去传入区神经元的存活和影响可塑性过程。CNTF缺陷小鼠的出生后神经元损失表明该因子在生理条件下也具有功能。在嗅球中，神经发生，轴突和突触发生持续整个生命，CNTF含量组成高。CNTF在大鼠嗅球中的细胞定位尚未完全解决，小鼠和大鼠之间的种属差异尚未确定。在本研究中，四种不同的CNTF抗体和双重免疫标记与胶质细胞和神经元细胞的特异性标记，研究CNTF在大鼠和小鼠嗅球的细胞定位。检测的特异性用来自CNTF-cleficient小鼠的组织检查，并且通过在CNTF启动子控制下合成β-半乳糖苷酶的小鼠（CNTF lacZ-敲入小鼠）中报告蛋白的免疫定位来补充调查。在这两个物种中，CNTF定位于成鞘细胞核，细胞体和轴突包裹过程。此外，个别轴突的嗅觉神经元的CNTF免疫反应。小鼠CNTF蛋白含量和免疫反应强度均低于大鼠。在颗粒层和外丛状层可见散在的CNTF阳性细胞。一些CNTF阳性细胞与神经细胞粘附分子的多唾液酸化形式的免疫反应性相关，所述神经细胞粘附分子由来自吻侧迁移流的成熟中间神经元表达。在CNTF lacZ基因敲入小鼠中，在嗅神经层的成鞘细胞以及肾小球、外网状和颗粒层的细胞中发现β-半乳糖苷酶反应性。研究证明CNTF定位于啮齿动物嗅球的神经胶质和神经元结构中。在小鼠中的结果提供了一个调查的基础上缺乏CNTF缺陷小鼠的因子的影响。(C)2003年IBRO。由Elsevier Science Ltd.出版，版权所有。
Ciliary neurotrophic factor (CNTF) is primarily regarded as an astrocytic lesion factor, promoting neuronal survival and influencing plasticity processes in deafferented areas of the CNS. Postnatal loss of neurons in CNTF-deficient mice indicates a function of the factor also under physiological conditions. In the olfactory bulb, where neurogenesis, axo- and synaptogenesis continue throughout life, CNTF content is constitutively high. The cellular localization of CNTF in the rat olfactory bulb is not fully resolved, and species differences between mouse and rat are not yet characterized. In the present study, four different CNTF antibodies and double immunolabeling with specific markers for glial and neuronal cells were used to study the cellular localization of CNTF in rat and mouse olfactory bulb. Specificity of the detection was checked with tissue from CNTF-cleficient mice, and investigations were complemented by immunolocalization of reporter protein in mice synthesizing beta-galactosidase under control of the CNTF promoter (CNTF lacZ-knock-in mice). In both species, CNTF localized to ensheathing cell nuclei, cell bodies and axon-enveloping processes. Additionally, individual axons of olfactory neurons were CNTF immunoreactive. Both CNTF protein content and immunoreaction intensity were lower in mice than in rats. Scattered lightly CNTF-reactive cells were found in the granular and external plexiform layers in rats. Some CNTF-positive cells were associated with immunoreactivity for the polysialylated form of the neural cell adhesion molecule, which is expressed by maturing interneurons derived from the rostral migratory stream. In CNTF lacZ-knock-in mice, beta-galactosidase reactivity was found in ensheathing cells of the olfactory nerve layer, and in cells of the glomerular, external plexiform and granular layers. The study proves that CNTF is localized in glial and neuronal structures in the rodent olfactory bulb. Results in mice provide a basis for investigations concerning the effects of a lack of the factor in CNTF-deficient mice. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.