Nucleolar localization of early tRNA processing
Nucleolar localization of early tRNA processing
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DOI：
10.1101/gad.12.16.2463
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发表时间：
1998-08-15
影响因子：
10.5
通讯作者：
Engelke, DR
中科院分区：
文献类型：
--
作者：
Bertrand, E;Houser-Scott, F;Engelke, DR
关于早期tRNA生物合成的位置信息很少。在芽殖酵母（Saccharomyces cerevisiae）中使用荧光原位杂交，显示核前体tRNA的实例主要存在于核仁中。我们还探测了RNase P的RNA亚基。RNase P探针的大部分信号是核仁的，在核质中的信号较弱。这些结果表明，tRNA加工途径的主要部分是在核仁与rRNA合成和核糖体组装区室化。空间上的并列表明tRNA和核糖体生物合成之间的直接协调的可能性。
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA subunit of RNase P. The majority of the signal from RNase P probes was nucleolar, with less intense signals in the nucleoplasm. These results demonstrate that a major portion of the tRNA processing pathway is compartmentalized in nucleoli with rRNA synthesis and ribosomal assembly. The spatial juxtaposition suggests the possibility of direct coordination between tRNA and ribosome biosynthesis.