Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor
Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor
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DOI：
10.1007/s00425-003-1136-y
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发表时间：
2004-02-01
期刊：
影响因子：
4.3
通讯作者：
Yamaoka, N
中科院分区：
文献类型：
--
作者：
Hagihara, T;Hashi, M;Yamaoka, N
表达丁香假单胞菌pv的细菌制备丁香内酯激发子。甘氨酸毒力基因D （avrD）仅诱导大豆（Glycine max）的超敏性细胞死亡（HCD）。携带Rpg4抗病基因的植物。采用差异展示法，从丁香内酯处理的大豆品种Harosoy （Rpg4）培养细胞中分离出13个基因片段。在rpg4品种Acme和Harosoy中，丁香内酯诱导了几个分离片段的基因；然而，7个被指定为SIH（丁香内酯诱导/HCD相关）片段的基因在哈罗索中被完全或强烈诱导。从丁香内酯处理的哈罗大豆cDNA文库中获得了SIH基因的cDNA克隆。一些序列与植物防御反应相关的蛋白质同源。SIH基因对非特异性β -葡聚糖激发子没有反应，而β -葡聚糖激发子会诱导植物抗毒素积累，但对HCD没有反应，这表明SIH基因的诱导是丁香内酯-哈罗索相互作用的特异性诱导。丁香内酯对HCD和SIH基因的诱导不受H2O2的影响。另一方面，Ca2+是HCD和一些SIH基因诱导所必需的。这些结果表明，丁香内酯诱导SIH基因可能通过丁香内酯特异性信号通路被激活，SIH基因产物可能在丁香内酯诱导的HCD过程中发挥重要作用。
Syringolide elicitors produced by bacteria expressing Pseudomonas syringae pv. glycinea avirulence gene D (avrD) induce hypersensitive cell death (HCD) only in soybean (Glycine max [L.] Merr.) plants carrying the Rpg4 disease resistance gene. Employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar Harosoy (Rpg4) treated with syringolides. Several genes for isolated fragments were induced by syringolides in an rpg4 cultivar Acme as well as in Harosoy; however, the genes for seven fragments designated as SIH (for syringolide-induced/HCD associated) were induced exclusively or strongly in Harosoy. cDNA clones for SIH genes were obtained from a cDNA library of Harosoy treated with syringolide. Several sequences are homologous to proteins associated with plant defense responses. The SIH genes did not respond to a non-specific beta-glucan elicitor, which induces phytoalexin accumulation but not HCD, suggesting that the induction of the SIH genes is specific for the syringolide-Harosoy interaction. HCD and the induction of SIH genes by syringolides were independent of H2O2. On the other hand, Ca2+ was required for HCD and the induction of some SIH genes. These results suggest that the induction of SIH genes by syringolides could be activated through the syringolide-specific signaling pathway and the SIH gene products may play an important role(s) in the processes of HCD induced by syringolides.