Splice Isoforms of Transcription Factor Elf-1 Affecting Its Regulatory Function in Transcription—Molecular Cloning of Rat Elf-1
Splice Isoforms of Transcription Factor Elf-1 Affecting Its Regulatory Function in Transcription—Molecular Cloning of Rat Elf-1
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转录因子Elf-1剪接异构体对其转录调节功能的影响——大鼠Elf-1的分子克隆
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
T. Yokota
中科院分区：
文献类型：
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作者：
C. Nishiyama;Kyoko Takahashi;M. Nishiyama;K. Okumura;C. Ra;Y. Ohtake;T. Yokota
为了阐明ELF-1在FcεRIα链表达中的作用，我们分离并鉴定了大鼠ELF-1的cDNAs。大鼠Elf-1基因全长2744个碱基，含有一个1848个碱基的开放阅读框。除了全长的大鼠Elf-1基因(命名为类型1)外，还分离到了两种剪接异构体。其中一种亚型缺失第85-120位氨基酸残基(类型2)，另一种亚型缺失85-175位氨基酸残基(类型3)。在人体组织中也观察到了类似的亚型。使用瞬时共表达系统过表达大鼠ELF-1(类型1)可抑制α链启动子活性。不同亚型的抑制活性不同，类型2的抑制活性低于类型1，类型3没有抑制作用。这一观察结果表明，Elf-1的每个亚型在其控制下的基因表达中扮演着不同的角色。
To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.
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DOI：
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发表时间：
1996
期刊：
Oncogene.
影响因子：
--
作者：
Miyazaki,Y;Sun,X;Uchida,H;Zhang,J;Nimer,S
通讯作者：
Nimer,S
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影响因子：
20.3
作者：
Nimer,SD;Zhang,W;Kwan,K;Whang,Y;Zhang,J;Wang,Y
通讯作者：
Wang,Y
影响因子：
56.9
作者：
WANG, CY;PETRYNIAK, B;LEIDEN, JM
通讯作者：
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影响因子：
20.3
作者：
Nimer,S;Zhang,J;Avraham,H;Miyazaki,Y
通讯作者：
Miyazaki,Y