DUAL BAR HOMEO BOX GENES OF DROSOPHILA REQUIRED IN 2 PHOTORECEPTOR CELLS, R1 AND R6, AND PRIMARY PIGMENT-CELLS FOR NORMAL EYE DEVELOPMENT
DUAL BAR HOMEO BOX GENES OF DROSOPHILA REQUIRED IN 2 PHOTORECEPTOR CELLS, R1 AND R6, AND PRIMARY PIGMENT-CELLS FOR NORMAL EYE DEVELOPMENT
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DOI：
10.1101/gad.6.1.50
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发表时间：
1992-01-01
影响因子：
10.5
通讯作者：
SAIGO, K
中科院分区：
文献类型：
--
作者：
HIGASHIJIMA, SI;KOJIMA, T;SAIGO, K
在果蝇的Bar突变中，已知小眼分化在眼睛的前部受到抑制。我们的结构分析表明，Bar区域包含一对同源框基因BarH1和BarH2。这些基因编码的多肽在大小和序列上相似，并且共享一个共同的同源结构域，除了假定的反式激活物结合位点外，该结构域在序列上是相同的。我们还通过嵌合分析和抗BarH1/BarH2抗体的免疫染色表明，BarH1和BarH2不仅在发育中的小眼的R1/R6前感光细胞和初级色素细胞中特异地共表达，而且在功能上也是必需的。在R1/R6中，BarH1和BarH2的表达似乎受Rough和Glass基因产物的调控。BarH1和BarH2蛋白对正常晶状体的形成、三种类型的色素细胞的形成以及从成熟的小眼膜中清除多余的细胞是必不可少的。综上所述，我们的结果表明，Bar同源域蛋白可能在色素细胞和锥体细胞的命运决定过程中发挥关键作用。
In the Bar mutation of Drosophila, ommatidial differentiation is known to be suppressed in the anterior portion of the eye. Our structural analysis shows that the Bar region contains a pair of homeo box genes, BarH1 and BarH2. These genes encode polypeptides similar in size and sequence and share a common homeo domain that is identical in sequence except for putative trans-activator-binding sites. We also show, by mosaic analysis and immunostaining with anti-BarH1/BarH2 antibodies, that BarH1 and BarH2 are not only specifically coexpressed but also functionally required in R1/R6 prephotoreceptors and primary pigment cells in developing ommatidia. In R1/R6, the expression of BarH1 and BarH2 appears to be regulated by rough and glass gene products. BarH1 and BarH2 proteins are essential to normal lens formation, formation of three types of pigment cells, and elimination of excess cells from mature ommatidia. Taken together, our results suggest that Bar homeo domain proteins may play key roles in the fate-determination processes of pigment cells and cone cells.