GC-MS determined cotinine in an epidemiological study on smoking status at delivery
GC-MS determined cotinine in an epidemiological study on smoking status at delivery
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DOI：
10.1016/j.pupt.2007.11.001
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发表时间：
2008-01-01
影响因子：
3.2
通讯作者：
Coudore, Francois
中科院分区：
文献类型：
--
作者：
de Chazeron, Ingrid;Daval, Sandrine;Coudore, Francois
本研究的目的是利用气相色谱-质谱（GC-MS）方法在流行病学分娩人群中测定孕妇及其新生儿的血浆可替宁水平，这些人群的烟草摄入量范围很大。本研究选取了来自各地区产科病房的近1000名孕妇（n = 1007）。每位患者都记录了吸烟日记，并接受了血液测试以评估可替宁的水平，同时还采集了新生儿的冠状血浆。然后对这些值进行交叉核对。可替宁采用选择离子监测模式，定量限为1.5 ng/ml。无论母亲的吸烟状况如何，母亲和新生儿的可替宁水平高度相关，活跃吸烟者的可替宁水平计算截止值为21.5 ng/ml。最后，通过气相色谱-质谱法测定的可替宁为孕妇及其婴儿的烟草暴露提供了灵敏、准确的测量方法。(C) 2007 Elsevier Ltd.版权所有。
The objective of this study was to measure the plasma cotinine levels in pregnant women and their newborns using a gas chromatography mass spectrometry (GC-MS) method in an epidemiological-delivered population with a wide range of tobacco intakes. Nearly 1000 pregnant women from regional maternity wards (n = 1007) were selected for the study. Each patient kept a tobacco diary and underwent a blood test to assess cotinine levels and at the same time that the newborns' cordonal plasma was taken. These values were then cross-checked.Cotinine was estimated using a selected-ion monitoring mode with a 1.5 ng/ml quantification limit. The cotinine levels in mothers and newborns were highly correlated, whatever the mother's smoking status, with a calculated cut-off for cotinine levels in active smokers of 21.5 ng/ml. Finally, the cotinine determined through this GC-MS method offered a sensitive and accurate measure of tobacco exposition of the pregnant women and their babies. (C) 2007 Elsevier Ltd. All rights reserved.