The effect of strenuous versus moderate exercise on the metabolism of proteoglycans in articular cartilage from different weight-bearing regions of the equine third carpal bone
The effect of strenuous versus moderate exercise on the metabolism of proteoglycans in articular cartilage from different weight-bearing regions of the equine third carpal bone
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DOI：
10.1016/s1063-4584(97)80011-8
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发表时间：
1997-05-01
影响因子：
7
通讯作者：
Rose, R
中科院分区：
文献类型：
--
作者：
Little, B;Ghosh, P;Rose, R
关节软骨退化中腕关节是一个常见的问题，在赛马。本研究评估了运动对关节软骨中大聚集蛋白聚糖（聚集蛋白聚糖）和两种小蛋白聚糖（双糖蛋白聚糖和核心蛋白聚糖）体外合成的影响，所述关节软骨取自进行中度或剧烈运动的马的第三腕骨的三个承重区域。对12匹无中腕关节临床和影像学疾病的Standardbred马进行8周中度运动计划。然后将马随机分为两组：A组-持续中等运动和B组-剧烈运动17周。然后让马休息16周。从第三腕骨的背侧桡关节面（DRF）、背侧中间关节面（DIF）和掌侧髁（PC）收集全深度关节软骨外植体并进行培养。测定软骨素蛋白聚糖含量和释放到培养基中。用（SO 42-）-S-35标记新合成的蛋白聚糖48 h，通过尺寸排阻和疏水层析、十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳（PAGE）和放射自显影进行分析。对相邻骨软骨组织切片进行评估，以确定关节炎变化的证据。在检查的任何关节软骨区域均未检测到组织学异常或蛋白多糖含量差异。然而，在来自B组动物的DRF的关节软骨中，聚集蛋白聚糖合成显著减少（P <0.05），同时核心蛋白聚糖合成增加（P < 0.05）。双糖蛋白聚糖合成、聚集蛋白聚糖流体动力学大小或在任何关节软骨区域聚集的能力没有变化。这项研究表明，剧烈运动的马可以导致在关节软骨区域蛋白多糖的生物合成受到高接触应力（DRF）的干扰。这些代谢异常在停止运动后持续16周，可能对组织的生物力学特性产生有害影响。我们认为，观察到的变化，在CRF软骨的剧烈运动的马的关节软骨代谢可能是一个诱发因素，软骨退化和骨关节炎在后期阶段。
Articular cartilage degeneration in the middle carpal joint is a common problem in racing horses. This study evaluated the effect of exercise on the in-vitro synthesis of the large aggregating proteoglycans (aggrecan) and two small proteoglycans, biglycan and decorin, in articular cartilage taken from three weight bearing regions of the third carpal bone of horses which were subjected to moderate or strenuous exercise. Twelve Standardbred horses free from clinical and radiographic disease of the middle carpal joint were subjected to an 8 week moderate exercise program. The horses were then randomly assigned to two groups: group A--continued moderate exercise and group B--strenuous exercise for 17 weeks. Horses were then rested for 16 weeks. Full-depth articular cartilage explants from the dorsal radial facet (DRF), dorsal intermediate facet (DIF) and palmar condyle (PC) of the third carpal bone were collected and cultured. Cartilage proteoglycan content and release into culture media were measured. Newly synthesized proteoglycans were labeled with (SO42-)-S-35 for 48 h and analyzed by size exclusion and hydrophobic chromatography, sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and autoradiography. Histologic sections of adjacent osteochondral regions were evaluated for evidence of arthritic change. No histologic abnormalities or differences in proteoglycan content were detected in any of the articular cartilage regions examined. There was however, a significant reduction (P < 0.05) in aggrecan synthesis and a concomitant increase in decorin synthesis (P < 0.05) in articular cartilage from the DRF of group B animals. There was no change in biglycan synthesis, aggrecan hydrodynamic size or ability to aggregate in any articular cartilage region. This study has demonstrated that strenuous exercise in horses can lead to a disturbance in the biosynthesis of proteoglycans in articular cartilage regions subjected to high contact stresses (DRF). These metabolic abnormalities, which persisted for 16 weeks after cessation of exercise, could have deleterious effects on the biomechanical properties of the tissue. We suggest that the observed alteration in articular cartilage metabolism in CRF cartilage of strenuously exercised horses could represent a predisposing factor for cartilage degeneration and osteoarthritis at a later stage.