Analysis of Fusarium graminearum mycotoxins in different biological matrices by LC/MS
Analysis of Fusarium graminearum mycotoxins in different biological matrices by LC/MS
复制标题
DOI：
10.1023/b:myco.0000012218.27359.ec
复制
发表时间：
2004-01-01
期刊：
影响因子：
5.5
通讯作者：
Regnault-Roger, C
中科院分区：
文献类型：
--
作者：
Bily, AC;Reid, LM;Regnault-Roger, C
本研究的目的是建立一种LC/MS检测方法，以准确地检测不同基质中的禾谷镰刀菌产生的三种真菌毒素。使用不同的LC条件，检测脱氧雪腐镰刀菌烯醇（DON），15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON），玉米赤霉烯酮（ZEN）在四个不同的基质（真菌液体培养物，玉米粒，昆虫幼虫和猪血清）。MS检测的灵敏度允许我们检测低至8 ppb的DON和12 ppb的ZEN的浓度。因此，成功分析这些毒素需要非常少量的基质，并且使用这种技术成功地研究了各种实验情况。在F.在受控条件下生长的禾谷镰刀菌。采用简单的提取方法，在接种不同的玉米基因型对F.禾谷早熟禾用含ZEN的人工饲料连续饲养玉米害虫进行毒代动力学研究，表明幼虫可能具有降解ZEN的能力。最后，在添加有DON的饲料中评估DON的持久性，结果表明DON在猪血液中快速积累，然后水平在12小时内进行性下降。本文报道的LC/MS研究对于在不同介质中和极低浓度下检测这些真菌毒素非常有用和灵活。
The purpose of this study was to develop an LC/MS assay to accurately detect three mycotoxins produced by Fusarium graminearum in various matrices. Using different LC conditions, deoxynivalenol ( DON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) were detected in four different matrices (fungal liquid cultures, maize grain, insect larvae and pig serum). The sensitivity of MS detection allowed us to detect concentrations as low as 8 ppb of DON and 12 ppb of ZEN. A very small quantity of matrix was therefore necessary for successful analysis of these toxins and a variety of experimental situations were successfully investigated using this technique. Production of 15-ADON and butenolide was monitored in a liquid culture of F. graminearum under controlled conditions. Using simple extraction procedures, the differential accumulation of DON and 15-ADON was followed in inoculated maize genotypes varying in susceptibility to F. graminearum. Toxicokinetic studies were carried out with maize insect pests reared continually on artificial diets containing ZEN and suggested that larvae may possess the ability to degrade ZEN. Finally, persistence of DON was assessed in pigs fed diet supplemented with DON, results indicated that DON accumulates quickly in pig blood and then levels decline progressively for 12 hours thereafter. The LC/MS study reported here is very useful and flexible for the detection of these mycotoxins in different media and at very low concentrations.