Domain Swap Approach Reveals the Critical Roles of Different Domains of SYMRK in Root Nodule Symbiosis in Lotus japonicus.
Domain Swap Approach Reveals the Critical Roles of Different Domains of SYMRK in Root Nodule Symbiosis in Lotus japonicus.
复制标题
结构域交换方法揭示了 SYMRK 不同结构域在百脉根根瘤共生中的关键作用
DOI：
10.3389/fpls.2018.00697
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li H;Chen M;Duan L;Zhang T;Cao Y;Zhang Z
共生受体激酶（Symbiosis receptor kinase，SYMRK）是一种定位于细胞膜上的蛋白激酶，含有胞外malectin-like domain（MLD）和leucine-rich repeat domain（LRR）结构域，是根瘤共生（root nodule symbiosis，RNS）和丛枝菌根共生（arbuscular mycorrhizal symbiosis，AMS）所必需的。SYMRK广泛分布于不同植物物种的基因组中，然而，SYMRK的不同结构域及其来自其他植物物种的同源物对RNS的贡献在很大程度上是不清楚的。本研究利用结构域交换技术，对蒺藜苜蓿（Medicago truncatula，RNS）、水稻（Oryza sativa，AMS）和拟南芥（Arabidopsis thaliana，RNS）的SYMRK及其同源基因进行了研究。来自水稻和苜蓿的全长SYMRK可以补充Lotus symrk-409突变体植物以贡献RNS，而来自拟南芥的全长SYMRK不能。苜蓿和水稻中的LjSYMRK的胞外结构域（艾德）和SYMRK的胞质结构域（CD）的嵌合蛋白可以在莲花中促进RNS，这表明SYMRK的CD是共生信号传导所必需的。含有LjSYMRK的CD（SYMRKCD）和MtDMI 2的ED（MtDMI 2 ED）、OsSYMRK的ED（OsSYMRKED）、AtSYMRK的ED（AtSYMRKED）、NFR 1的ED（NFR 1 ED）和NFR 5的ED（NFR 5ED）的嵌合受体可以补充Lotus symrk-409突变体植物以产生根瘤。然而，MtDMI 2可以部分地与Lotus symrk-409突变体互补，形成有效结节和无效隆起，这与MtDMI 2 ED-LjSYMRKCD和LjSYMRKGDLC在Lotus symrk-409突变体中的互补结果相似，表明SYMRK的艾德对Lotus的RNS具有非常精细的调节作用。SYMRKGDLC（SYMRK的GDPC基序被GDLC取代的突变体）上的MLD或LRR的缺失在Lotus symrk-409突变体中过表达时可能有助于RNS，这表明MLD和LRR结构域可能共同参与共生信号传导，并且LRR结构域可能在LjSYMRKGDLC介导的RNS中起负面作用。通过对LRR结构域保守氨基酸的突变，发现LjSYMRKGDLC在RNS中的功能需要5个丝氨酸残基。这些发现精确地阐明了SYMRK在L. - 是的
Symbiosis receptor kinase (SYMRK) is a cell membrane-localized protein kinase containing extracellular malectin-like domain (MLD) and leucine-rich repeat (LRR) domains, which is critically required for both root nodule symbiosis (RNS) and arbuscular mycorrhizal symbiosis (AMS). SYMRK is widely distributed in the genomes of different plant species; however, the contribution of different domains of SYMRK and its homologs from other plant species to RNS is largely unclear. In this study, SYMRK and its homologs from three typical plant species including Medicago truncatula (for both RNS and AMS), Oryza sativa (for AMS but not RNS), and Arabidopsis thaliana (for neither RNS or AMS) were investigated using domain swap approach in response to rhizobia in Lotus japonicus. Full-length SYMRK from rice and Medicago but not from Arabidopsis could complement Lotus symrk-409 mutant plants to contribute RNS. The chimeric protein with the extracellular domain (ED) of LjSYMRK and cytoplasmic domains (CD) of SYMRK from both Medicago and rice but not Arabidopsis could contribute to RNS in Lotus, suggesting that the CD of SYMRK is required for symbiotic signaling. The chimeric receptors containing the CD of LjSYMRK (SYMRKCD) and the EDs of MtDMI2 (MtDMI2ED), OsSYMRK (OsSYMRKED), AtSYMRK (AtSYMRKED), NFR1 (NFR1ED), and NFR5 (NFR5ED) could complement Lotus symrk-409 mutant plants to develop nodules. However, MtDMI2 could partially complement Lotus symrk-409 mutants to form both effective nodules and ineffective bumps, which is similar to the complementation results from MtDMI2ED-LjSYMRKCD and LjSYMRKGDLC in Lotus symrk-409 mutants, suggesting that ED of SYMRK has a very fine-tune regulation for RNS in Lotus. The deletion of either MLD or LRR on SYMRKGDLC (a mutant version of SYMRK with GDPC motif replaced by GDLC) could contribute to RNS when overexpressed in Lotus symrk-409 mutants, suggesting that MLD and LRR domains might work together to be involved in symbiotic signaling and the LRR domain might play a negative role in LjSYMRKGDLC-mediated RNS. By mutagenizing the conserved amino acids on LRR domain, five serine residues were found to be required for the function of LjSYMRKGDLC in RNS. These finding precisely refine the molecular mechanisms of SYMRK function in symbiotic signaling in L. japonicus.
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影响因子：
7.7
作者：
Ried MK;Antolín-Llovera M;Parniske M
通讯作者：
Parniske M
影响因子：
16.6
作者：
Kawaharada Y;Nielsen MW;Kelly S;James EK;Andersen KR;Rasmussen SR;Füchtbauer W;Madsen LH;Heckmann AB;Radutoiu S;Stougaard J
通讯作者：
Stougaard J
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
7.4
作者：
Arrighi, Jean-Francois;Barre, Annick;Gough, Clare
通讯作者：
Gough, Clare
影响因子：
9.2
作者：
Antolin-Llovera, Meritxell;Ried, Martina K.;Parniske, Martin
通讯作者：
Parniske, Martin