Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius.
Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius.
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拟杆菌属物种的超微结构：Bacteroides asaccharolyticus、Bacteroides fragilis、Bacteroides melaninogenicus subspecies melaninogenicus 和 B. melaninogenicus subspecies intermedius。
DOI：
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发表时间：
1979
影响因子：
6.4
通讯作者：
E. R. Leadbetter
中科院分区：
文献类型：
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作者：
D. D. L. Woo;D. D. L. Woo;S. C. Holt;S. C. Holt;E. R. Leadbetter;E. R. Leadbetter
采用戊二醛锇固定法、钌红固定法和染色法对从人直肠周围脓肿分离的2个亚种黑素拟杆菌（melaninogenicus亚种和intermedius亚种）、溶糖拟杆菌（Bacteroides asaccharolyticus）、溶糖拟杆菌（B. asaccharolyticus）菌株536B和脆弱拟杆菌(Bacteroides fragilis ATCC 25285的代表性菌株进行检测。和羟基钍染色以及临界点干燥-透射电子显微镜（CPD- TEM）和扫描电子显微镜（SEM）物理制备技术。除脆弱芽孢杆菌25285外，所有菌株的外膜外均含有电子致密物质。钌红染色进一步揭示了外膜表面外的一层，对每个被检查的物种来说都是不同的。氢氧化钍以及CPD- TEM和SEM显示，细胞之间通过细纤维相互连接，这些细纤维不仅连接相邻的细胞，而且穿过几微米连接细胞聚集体。
Representative strains of two subspecies of Bacteroides melaninogenicus (subspecies melaninogenicus and subspecies intermedius) and Bacteroides asaccharolyticus as well as B. asaccharolyticus strain 536B isolated from a human perirectal abscess and Bacteroides fragilis ATCC 25285 were examined by glutaraldehyde-osmium fixation, ruthenium red fixation and staining, and thorium hydroxyde staining as well as by the physical preparative techniques of critical point drying--transmission electron microscopy (CPD--TEM) and scanning electron microscopy (SEM). All strains, with the exception of B. fragilis 25285, possessed an electron-dense material external to their outer membranes. Ruthenium red staining further revealed a layer, external to the surface of the outer membrane, that was distinct for each species examined. Thorium hydroxide, as well as CPD--TEM and SEM, showed the cells to be interconnected by thin fibers that not only connected adjacent cells but also traversed several microns to connect cell aggregates.