Buffers, Especially the Good Kind
Buffers, Especially the Good Kind
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DOI：
10.1021/acs.biochem.1c00200
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发表时间：
2021-05-03
期刊：
影响因子：
2.9
通讯作者：
Pielak, Gary J.
中科院分区：
文献类型：
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作者：
Pielak, Gary J.
55年前，诺曼·古德和他的同事们撰写了一篇论文，从根本上推进了湿生物化学[古德，N。E、Winget，G. D、温特，W.，康诺利，T。N.，Izawa，S.，和Singh，R. M. M.（1966）用于生物研究的氢离子缓冲剂。生物化学5，467-477]，并在这样做已经积累了超过2500引文。他们列出了有用的、与生物化学相关的氢离子缓冲剂所需的特性，然后合成并测试了其中的10种。不久之后，这些缓冲液就可以在市场上买到了。从那以后，我们大多数人从来没有想过他们。我们只是使用它们。在这里，我讨论了一些关于“好的缓冲液”的起源的背景，对非好的缓冲液，三羟甲基氨基甲烷，做了一些观察，并建议我们通过结合好等人的想法来合成新的缓冲液。
Fifty-five years ago, Norman Good and colleagues authored a paper that fundamentally advanced wet biochemistry [Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., and Singh, R. M. M. (1966) Hydrogen ion buffers for biological research. Biochemistry 5, 467-477] and in doing so has amassed more than 2500 citations. They laid out the properties required for useful, biochemically relevant hydrogen-ion buffers and then synthesized and tested 10 of them. Soon after, these buffers became commercially available. Since then, most of us never gave them a second thought. We just use them. Here, I discuss some of the background regarding the genesis of "Good's buffers", make a few (disparaging) observations about the non-Good's buffer, Tris, and suggest that we synthesize new buffers by combining the ideas of Good et al. with results from the past 60 years of protein chemistry.