Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength
Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength
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DOI：
10.1117/1.3463479
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发表时间：
2010-07-01
影响因子：
3.5
通讯作者：
Alfano, Robert R.
中科院分区：
文献类型：
--
作者：
Pu, Yang;Wang, Wubao;Alfano, Robert R.
测量了人前列腺癌组织和正常前列腺组织在340 nm选择性激发波长下的荧光光谱。采用交替最小二乘多元曲线分辨法研究了主要生化成分对组织荧光光谱的贡献。结果表明，与正常组织相比，癌组织中胶原蛋白的释放贡献减少，而烟酰胺腺嘌呤二核苷酸（NADH）的贡献增加。这种差异是由于在肿瘤发生发展过程中NADH和胶原相对含量的变化所致。这项研究可能为前列腺癌检测提供潜在的天然生物标志物。(C)2010年，美国光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）[DOI：10.1117/1.3463479]
The fluorescence spectra of human cancerous and normal prostate tissues obtained by the selective excitation wavelength of 340 nm were measured. The contributions of principle biochemical components to tissue fluorescence spectra were investigated using the method of multivariate curve resolution with alternating least squares. The results show that there is a reduced contribution from the emission of collagen and increased contribution from nicotinamide adenine dinucleotide (NADH) in cancerous tissues as compared with normal tissue. This difference is attributed to the changes of relative contents of NADH and collagen during cancer development. This research may present a potential native biomarker for prostate cancer detection. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3463479]