Integrative treatment of anti-tumor/bone repair by combination of MoS2 nanosheets with 3D printed bioactive borosilicate glass scaffolds
Integrative treatment of anti-tumor/bone repair by combination of MoS2 nanosheets with 3D printed bioactive borosilicate glass scaffolds
复制标题
MoS2纳米片与3D打印生物活性硼硅酸盐玻璃支架组合的抗肿瘤/骨修复综合治疗
DOI：
10.1016/j.cej.2020.125081
复制
发表时间：
2020
影响因子：
15.1
通讯作者：
Li Jiusheng
中科院分区：
文献类型：
--
作者：
Wang Hui;Zeng Xiangqiong;Pang Libin;Wang Haihang;Lin Bocai;Deng Zhengwei;Qi Edwina Lau Xiu;Miao Na;Wang Deping;Huang Peng;Hu Haoran;Li Jiusheng
恶性骨肿瘤切除后肿瘤复发、大面积缺损难以重建和修复，给骨肿瘤的治疗带来了极大的障碍和严重的疾病。此外，在生物医学领域中，抗肿瘤剂的长期功效、令人满意的生物相容性和优异的性质是必需的。针对这些问题，提出了在硼硅酸盐生物活性玻璃（BG）表面覆盖光热疗法（PTT）复合MoS 2-PLGA膜，构建一体化抗肿瘤/骨修复支架的新思路。在我们的研究中，二硫化钼集成复合BG（BGM）支架可以快速，有效地升温，他们表现出良好的光热稳定性，在808 nm的激光照射下。值得注意的是，BGM支架可以有效地降低体外骨肉瘤细胞（MNNG/HOS）的活力，并抑制裸鼠肿瘤的生长。此外，所制备的BGM支架可以刺激大鼠骨髓间充质干细胞（rBMSCs）的增殖和分化，在体外上调成骨相关基因（Runx-2，OCN和Col-I）的表达，并促进在体内骨修复大鼠颅骨缺损的临界尺寸。因此，新型的MoS 2-整合的复合BG支架在治疗肿瘤相关的骨缺损方面具有很大的前景，为组织工程领域新材料的制造提供了思路。
Malignant bone tumors have caused great obstacles and serious illnesses for tumor recurrence and difficulty in reconstructing and repairing large defects after tumorectomy. Additionally, long-term efficacy, satisfactory biocompatibility and excellent properties for anti-tumor agents are necessary in the biomedical field. To solve these problems, a novel idea has been proposed on building an integrative anti-tumor/bone repairing scaffold by covering photothermal therapy (PTT) composite MoS2-PLGA film on the surface of borosilicate bioactive glass (BG). In our study, the MoS2-integrated composite BG (BGM) scaffolds can rapidly and effectively elevate temperature, and they exhibited excellent photothermal stability, under 808 nm laser irradiation. Notably, the BGM scaffolds can effectively reduce the viability of osteosarcoma cells (MNNG/HOS) in vitro as well as inhibit the tumor growth in nude mice. Furthermore, the prepared BGM scaffolds can stimulate the proliferation and differentiation of rat bone mesenchymal stem cells (rBMSCs), upregulate the expression of osteogenesis-related genes (Runx-2, OCN and Col-I) in vitro and promote in vivo bone repair in critical-sized rat calvarial defects. Therefore, the novel MoS2-integrated composite BG scaffolds are highly promising for the treatment of tumor-related bone defects, offering ideas for the manufacture of new materials in the tissue engineering field.