Defective repair of radiation-induced chromosomal damage in scid/scid mice.
Defective repair of radiation-induced chromosomal damage in scid/scid mice.
复制标题
scid/scid 小鼠中辐射诱导的染色体损伤的缺陷修复。
DOI：
10.1159/000133196
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Shultz,LD
中科院分区：
文献类型：
--
作者：
Disney,JE;Barth,AL;Shultz,LD
小鼠严重联合免疫缺陷(SCID)突变干扰免疫球蛋白和T细胞受体基因的正常重组。这种免疫缺陷导致在SCID/SCRIDD小鼠体内缺乏完全分化的B和T细胞。这种突变纯合子的动物对电离辐射的损害也表现出更高的敏感度。我们在此报告了我们在SCID/SCID小鼠的骨髓细胞和成纤维细胞中观察到的高频率的辐射诱导的染色单体互换和胞内变化。这些异常染色体结构的存在表明，链重新连接的延迟是SCID/SCID小鼠对电离辐射敏感性增加的基础。这种突变可能为理解高等真核细胞中有丝分裂重组与DNA修复之间的关系提供重要线索。
The murine severe combined immunodeficiency (scid) mutation interferes with normal recombination of iramunoglobulin and T-cell receptor genes. This immunologic defect results in a lack of fully differentiated B and T cells inscid/scidmice. Animals homozygous for thescidmutation also display increased sensitivity to the damaging effects of ionizing radiation. We report here our observations of high frequencies of radiation-induced chromatid interchanges and intrachanges in bone marrow cells and fibroblasts fromscid/scidmice. The presence of these aberrant chromosome structures suggests that a delay in strand rejoining underlies the increased sensitivity ofscid/scidmice to ionizing radiation. Thescidmutation may provide important clues for understanding the relationship between mitotic recombination and DNA repair in higher eukaryotic cells.