Extended chloramination significantly enriched intracellular antibiotic resistance genes in drinking water treatment plants.
Extended chloramination significantly enriched intracellular antibiotic resistance genes in drinking water treatment plants.
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DOI：
10.1016/j.watres.2023.119689
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发表时间：
2023-02
期刊：
影响因子：
12.8
通讯作者：
Hai-bei Li;Hongling Yu;Yongbing Liang;Xudong Zhang;Dong Yang;Lin Wang;Dan-yang Shi;Tianjiao Chen;Shu-qing Zhou;Jing Yin;Zhong-wei Yang;Jun-Wen Li;Min Jin
中科院分区：
文献类型：
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作者：
Hai-bei Li;Hongling Yu;Yongbing Liang;Xudong Zhang;Dong Yang;Lin Wang;Dan-yang Shi;Tianjiao Chen;Shu-qing Zhou;Jing Yin;Zhong-wei Yang;Jun-Wen Li;Min Jin
氯胺化和氯化都是防止潜在病原体通过饮用水传播给人类的强大屏障。然而，氯胺化和氯化对饮用水处理厂(DWTP)中抗生素耐药基因(ARGs)发生的比较影响尚不清楚。本研究通过对氯胺化或氯化前后水中抗生素耐药性进行元基因组测序，并通过实时定量聚合酶链式反应(QPCR)进行验证。处理90min后，氯胺化处理导致水中细胞内精氨酸(IARGs)的总相对丰度(IARGs)比氯化处理更高，而氯化处理比氯胺化处理更有利于胞外Args(EARGs)的释放。根据冗余度和皮尔逊分析，出厂水中观测到的iARGs总浓度与氨氮(NH4+-N)浓度呈显著正相关，并随着NH4+-N浓度的增加呈线性上升趋势。这表明，NH4+-N是氯胺化过程中iARG积累的重要驱动因素。如果氯胺化时间缩短到40分钟，iARG浓缩就会停止，这表明缩短持续时间将是控制饮用水中iARG浓缩的更好策略。这些发现强调了延长氯胺化作用后抗生素耐药性的潜在风险，有助于通过优化污水处理厂的消毒程序来控制抗药性细菌通过水传播。
Chloramination and chlorination are both strong barriers that prevent the transmission of potential pathogens to humans through drinking water. However, the comparative effects of chloramination and chlorination on the occurrence of antibiotic resistance genes (ARGs) in drinking water treatment plants (DWTPs) remain unknown. Herein, the antibiotic resistome in water before and after chloramination or chlorination was analyzed through metagenomic sequencing and then verified through quantitative real-time polymerase chain reaction (qPCR). After the treatment of 90 min, chloramination led to higher enrichment of the total relative abundance of intracellular ARGs (iARGs) in water than chlorination, whereas chlorination facilitated the release of more extracellular ARGs (eARGs) than chloramination. According to redundancy and Pearson's analyses, the total concentration of the observed iARGs in the finished water exhibited a strong positive correlation with ammonium nitrogen (NH4+–N) concentration, presenting a linear upward trend with an increase in the NH4+–N concentration. This indicated that NH4+–N is a crucial driving factor for iARG accumulation during chloramination. iARG enrichment ceases if the duration of chloramination is shortened to 40 min, suggesting that shortening the duration would be a better strategy for controlling iARG enrichment in drinking water. These findings emphasized the potential risk of antibiotic resistance after extended chloramination, shedding light on the control of transmission of antibiotic-resistant bacteria through water by optimizing disinfection procedures in DWTPs.