New developments in comet-FISH.
New developments in comet-FISH.
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彗星FISH的新进展。
DOI：
10.1093/mutage/geu036
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发表时间：
2015
期刊：
影响因子：
2.7
通讯作者：
Spivak,Graciela
中科院分区：
文献类型：
--
作者：
Spivak,Graciela
彗星试验结合荧光原位杂交（FISH）是一个强大的技术比较分析的损伤诱导和修复的基因组和特定的DNA序列在单细胞内。彗星FISH方法的最新进展将在这里被考虑，特别注意荧光探针的设计和生成。一般来说，所有的方法都必须满足一些基本要求：探针的长度应该不超过~300个核苷酸（单链或双链），以便能够穿透其中嵌入靶基因组DNA的凝胶，它们应该是序列特异性的，并且它们的信号应该是可检测的，并且与背景荧光和用于染色DNA的染料不同。
The comet assay combined with fluorescencein-situhybridisation (FISH) is a powerful technique for comparative analyses of damage induction and repair in genomes and in specific DNA sequences within single cells. Recent advances in the methodology of comet-FISH will be considered here, with particular attention to the design and generation of fluorescent probes. In general, all the approaches must fulfil a few basic requirements: the probes should be no longer than ~300 nucleotides in length (single or double stranded) to be able to penetrate the gel in which the target genomic DNA is embedded, they should be sequence-specific, and their signal should be detectable and distinct from the background fluorescence and the dye used to stain the DNA.