Involvement of Kv4.1 K+ Channels in Gastric Cancer Cell Proliferation
Involvement of Kv4.1 K+ Channels in Gastric Cancer Cell Proliferation
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DOI：
10.1248/bpb.33.1754
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发表时间：
2010-10-01
影响因子：
2
通讯作者：
Lee, So Yeong
中科院分区：
文献类型：
--
作者：
Kim, Hyung-Jin;Jang, Soo Hwa;Lee, So Yeong
电压门控钾通道不仅在可兴奋细胞中表达，而且在非可兴奋细胞如上皮细胞中也表达。最近的研究表明，几种亚型的Kv通道在上皮性肿瘤细胞（包括人胃癌细胞）中表达，并与细胞增殖相关。在本研究中，我们研究了Kv4.1在人胃癌细胞系中的表达以及抑制Kv4.1表达对细胞增殖和细胞周期分布的影响。我们发现Kv4.1 mRNA和蛋白在人胃癌细胞系MKN-45和SNU-638中表达。此外，Kv4.1靶向的小干扰RNA（siRNA）治疗抑制胃癌细胞增殖。流式细胞仪分析显示，抑制Kv4.1的表达诱导细胞周期进程的G1-S转换块。这些结果表明Kv4.1在人胃癌细胞系MKN-45和SNU-638的增殖中起作用，并且可以被认为是人胃癌的治疗靶点。
Voltage-gated potassium (Kv) channels are expressed not only in excitable cells but also in non-excitable cells such as epithelial cells. Recent studies have demonstrated that several subtypes of Kv channels are expressed in epithelial tumor cells, including human gastric cancer cells, and are associated with cell proliferation. In the present study, we examined the expression of Kv4.1 in human gastric cancer cell lines and the effects of suppressed expression of Kv4.1 on cell proliferation and cell cycle distribution. We found that Kv4.1 mRNA and protein are expressed in the human gastric cancer cell lines MKN-45 and SNU-638. Moreover, Kv4.1-targeted small interference RNA (siRNA) treatment inhibited gastric cancer cell proliferation. Flow cytometric analysis revealed that suppressed expression of Kv4.1 induced a G1-S transition block of cell cycle progression. These results reveal that Kv4.1 plays a role in the proliferation of the human gastric cancer cell lines MKN-45 and SNU-638 and can be considered as a therapeutic target for human gastric cancer.