Morphological Observation of Sympathetic Nerve Fibers in the Human Posterior Longitudinal Ligament
Morphological Observation of Sympathetic Nerve Fibers in the Human Posterior Longitudinal Ligament
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DOI：
10.1097/brs.0000000000000647
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发表时间：
2014-12-15
期刊：
影响因子：
3
通讯作者：
Chen, Bohua
中科院分区：
文献类型：
--
作者：
Li, Haiyan;Ma, Xuexiao;Chen, Bohua
研究设计.人类活体组织的组织学研究。确定颈椎前路减压手术患者颈椎后纵韧带（PLL）中的交感神经纤维，并推测其存在和分布的意义。颈椎病的发病机制尚不清楚。颈性眩晕常与耳源性眩晕、中枢性眩晕等混淆，人们逐渐认识到椎动脉的机械性干扰并不是解释颈性眩晕发病机制的唯一途径。应该注意的是，交感神经因素也可能涉及它，因为在椎间盘的动物研究中，在PLL中发现了一些交感神经。虽然目前还不清楚是否有一个类似的现象，在成人PLL。选择2013年1月至2013年12月在青岛大学附属医院行颈椎前路减压术的患者46例，分为颈椎病组和颈椎外伤组。所有受试者均在手术过程中获得颈椎后纵韧带组织。取韧带石蜡切片，用乙醛酸诱导荧光法染色。通过对不同切片上表达的荧光单位的测量和分析，观察交感神经纤维的形态和分布。对荧光染色阳性率进行统计学分析。从46例标本中选取每组标本，在三维切片上观察不同形态的交感神经纤维分布。颈椎病组与颈椎外伤组后纵韧带荧光单位阳性率无显著性差异（x（2）= 0.969，P > 0.05）。证实人颈椎后纵韧带内有交感神经纤维分布。
Study Design. Histological study of human living tissue.Objective. To determine sympathetic fiber in the cervical posterior longitudinal ligaments (PLL) obtained from the patients undergoing anterior cervical decompression surgery, and speculate the implication of their presence and distribution.Summary of Background Data. The pathogenic mechanism responsible for cervical spondylosis remains unclear. Cervical vertigo is often confused with aural vertigo, and central vertigo, and et al. It has been gradually realized that mechanical interference to the vertebral artery is not the only way to explain the pathogenic mechanism of cervical vertigo. It should be noted that the sympathetic factor may also involve it because some sympathetic nerves were found in the PLL in an animal study of intervertebral discs. Although it is unclear whether there is a similar phenomenon in adult human PLL.Methods. Forty-six patients who received anterior cervical decompression surgery in The Affiliated Hospital of Qingdao University from January 2013 to December 2013 were classified into 2 groups: with cervical spondylosis and with cervical trauma. Cervical PLL tissues of all the participants were obtained during operation. The paraffin slices of the ligament were stained according to glyoxylic acid-induced fluorescent method. The morphology and distribution of sympathetic nerve fibers were observed by measuring and analyzing fluorescent units expressed on different sections. The positive rates expressed by fluorescent staining were statistically analyzed.Results. Different forms of sympathetic nerve fibers distribution were observed in the 3-dimensional slices in each group selected from 46 cases of specimens. The positive rate of fluorescent units detected from the cervical PLL in patients experiencing cervical spondylosis was not significantly different from that in cervical trauma group (x(2) = 0.969, P > 0.05).Conclusion. Sympathetic nerve fibers were confirmed to distribute in the human cervical posterior longitudinal ligament.