Visfatin and oxidative stress influence endothelial progenitor cells in obese populations
Visfatin and oxidative stress influence endothelial progenitor cells in obese populations
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DOI：
10.3109/07435800.2014.952016
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Song, Guangyao
中科院分区：
文献类型：
--
作者：
Chen, Shuchun;Sun, Lina;Song, Guangyao
研究目的：本研究旨在研究肥胖个体循环内皮祖细胞（EPCs）、血清内脂素和氧化应激水平及其相关性。材料与方法：采用流式细胞术检测CD309和CD34，测定外周血EPCs水平。采用酶联免疫吸附法测定31例肥胖男性的血清内脂素浓度[肥胖组：BMI 28.9 +/- 0.86 kg/m；年龄44.93 +/- 1.78岁（40 ~ 47岁），正常体重男性30例[对照组：BMI 22.7 +/- 1.22 kg/m(2)；年龄44.03±1.87岁（41 ~ 47岁）。用比色法测定氧化应激指标。采用多元线性回归分析循环EPCs、血清visfatin和氧化应激标志物之间的关系。统计学意义设为p
Purpose/Aims of the study: This study sought to study the levels of circulating endothelial progenitor cells (EPCs), serum visfatin, and oxidative stress in obese individuals, and their respective correlations. Materials and methods: The circulating levels of EPCs were measured through detecting CD309 and CD34 by flow cytometry. Serum visfatin concentration was measured by enzyme-linked immunosorbent assay in 31 obese men [obese group: BMI 28.9 +/- 0.86 kg/m(2); age 44.93 +/- 1.78 years (range 40 to 47)] and 30 normal-weight men [control group: BMI 22.7 +/- 1.22 kg/m(2); age 44.03 +/- 1.87 years (range 41 to 47)]. Indexes of oxidative stress were assayed by a colorimetric method. The relationships between circulating EPCs, serum visfatin, and oxidative stress markers were further analyzed by multiple linear regression analysis. Statistical significance was set at p