A simple approach to obtain hybrid Au-loaded polymeric nanoparticles with a tunable metal load
A simple approach to obtain hybrid Au-loaded polymeric nanoparticles with a tunable metal load
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DOI：
10.1039/c5nr06850a
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发表时间：
2016-01-01
期刊：
影响因子：
6.7
通讯作者：
Santamaria, Jesus
中科院分区：
文献类型：
--
作者：
Luque-Michel, Edurne;Larrea, Ane;Santamaria, Jesus
报道了一种制备多功能聚合物-金属杂化纳米结构的新方法。通过使用该方案，克服了目前大多数关于聚合物与预形成的无机纳米颗粒（NP）的共价和非共价连接的发展的障碍。该策略是基于使用聚合物纳米颗粒作为纳米反应器的金属前体的原位还原。金纳米颗粒和聚（DL-乳酸-共-乙醇酸），PLGA，分别位于核和壳。这种新技术能够生产小于200 nm的PLGA NP，其承载单个封装的Au NP或具有可调尺寸和100%负载效率的几个较小的NP。聚合物纳米颗粒内部的Au离子的原位还原是通过使用热来激活柠檬酸根离子的还原作用而按需实现的。此外，我们表明，负载的PLGA纳米颗粒内的所得Au纳米颗粒是高度依赖于所使用的表面活性剂。电子显微镜，激光照射，紫外-可见和荧光光谱表征技术证实了Au纳米粒子的位置。这些有希望的结果表明，这些杂化纳米材料可用于治疗诊断应用或作为暗场成像和计算机断层扫描中的造影剂。
A new strategy to nanoengineer multi-functional polymer-metal hybrid nanostructures is reported. By using this protocol the hurdles of most of the current developments concerning covalent and non-covalent attachment of polymers to preformed inorganic nanoparticles (NPs) are overcome. The strategy is based on the in situ reduction of metal precursors using the polymeric nanoparticle as a nanoreactor. Gold nanoparticles and poly(DL-lactic-co-glycolic acid), PLGA, are located in the core and shell, respectively. This novel technique enables the production of PLGA NPs smaller than 200 nm that bear either a single encapsulated Au NP or several smaller NPs with tunable sizes and a 100% loading efficiency. In situ reduction of Au ions inside the polymeric NPs was achieved on demand by using heat to activate the reductive effect of citrate ions. In addition, we show that the loading of the resulting Au NPs inside the PLGA NPs is highly dependent on the surfactant used. Electron microscopy, laser irradiation, UV-Vis and fluorescence spectroscopy characterization techniques confirm the location of Au nanoparticles. These promising results indicate that these hybrid nanomaterials could be used in theranostic applications or as contrast agents in dark-field imaging and computed tomography.