Evaluation of oocyte quality: Morphological, cellular and molecular predictors
Evaluation of oocyte quality: Morphological, cellular and molecular predictors
复制标题
DOI：
10.1071/rd06103
复制
发表时间：
2007-01-01
影响因子：
1.9
通讯作者：
Sun, Qing-Yuan
中科院分区：
文献类型：
--
作者：
Wang, Qiang;Sun, Qing-Yuan
越来越多的证据表明，卵母细胞的质量深刻地影响受精和随后的胚胎发育，推动了对卵母细胞发育能力的可靠预测的持续寻找。在本综述中，我们对可用于评估卵母细胞质量的潜在标准进行了全面总结和分析。这些标准具体分类为形态学和细胞/分子预测因子。评价卵母细胞质量的传统方法是基于卵泡、卵丘-卵母细胞复合体、极体和/或减数分裂纺锤体的形态学分类。虽然使用形态学特征作为卵母细胞质量的预测因子是有争议的，但这样的分级系统可以为具有较高发育能力的卵母细胞的预选提供有价值的信息，因此可以最大限度地提高胚胎发育结果。一些内在标志物（如线粒体状态和葡萄糖-6-磷酸脱氢酶1活性）和外在标志物（如卵泡液或血清中卵泡细胞的凋亡和转化生长因子-β超家族的水平）也被报道为卵母细胞能力和胚胎质量的有用指标。与形态学参数相比，这些卵母细胞质量的细胞和分子预测指标可能会被证明更加准确和客观，尽管还需要进一步的研究和技术改进。
Mounting evidence that oocyte quality profoundly affects fertilisation and subsequent embryo development drives the continued search for reliable predictors of oocyte developmental competence. In the present review, we provide an overall summary and analysis of potential criteria that can be used to evaluate oocyte quality. These criteria are specifically classified as morphological and cellular/molecular predictors. Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and/or meiotic spindle. Although the use of morphological characteristics as predictors of oocyte quality is controversial, such a grading system can provide valuable information for the preselection of oocytes with higher developmental competence and, therefore, may maximise embryo developmental outcome. Several intrinsic markers (such as mitochondrial status and glucose-6-phosphate dehydrogenase 1 activity) and extrinsic markers (such as apoptosis of follicular cells and levels of the transforming growth factor-beta superfamily in follicular fluid or serum) have also been reported as useful indicators of oocyte competence and embryo quality. Compared with the morphological parameters, these cellular and molecular predictors of oocyte quality may prove to be more precise and objective, although further studies and refinement of techniques are needed.