Moesin overexpression is a unique biomarker of adenomyosis.
Moesin overexpression is a unique biomarker of adenomyosis.
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Moesin 过度表达是子宫腺肌病的独特生物标志物。
DOI：
10.1111/pin.12148
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Noguchi M.
中科院分区：
文献类型：
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作者：
Ohara R;Michikami H;Nakamura Y;Sakata A;Sakashita S;Satomi K;Shiba-Ishii A;Kano J;Yoshikawa H;Noguchi M.
子宫腺肌症的特征是子宫内膜腺体和间质细胞延伸到肌层。在这里，我们证明了“moesin”是子宫腺肌症的独特生物标志物。我们选择了两例手术切除并用福尔马林固定的子宫腺肌症病例。通过组织显微解剖从浸润性子宫腺肌症病变和正常子宫内膜中提取蛋白质。采用LC - MS/MS系统检测提取的蛋白，并比较各区域的表达谱。检测到260种蛋白，其中73种在子宫腺肌症中表达高于正常子宫内膜。在这些蛋白中，我们重点研究了moesin在子宫腺肌症中的过表达。使用免疫组织化学评分半定量估计moesin在子宫腺肌症中的表达高于正常子宫内膜。其中，moesin在子宫腺肌症间质细胞中的表达明显高于正常子宫内膜间质细胞。与正常子宫内膜相比，14例子宫腺肌症中有9例在RNA水平上过度表达moesin， 14例中均在蛋白水平上过度表达moesin。我们还在子宫腺肌症病变中检测到活化的（磷酸化的）moesin。目前的研究结果表明，moesin在子宫腺肌症中具有特征性的过表达和激活，并且moesin的激活可能与肌层子宫腺肌症的延伸有关。
Adenomyosis is characterized by extension of endometrial glands and stromal cells into the myometrium. Here we proved that ‘moesin’ is a unique biomarker of adenomyosis. We selected two cases of adenomyosis that had been surgically resected and fixed with formalin. Proteins were extracted from the infiltrating adenomyosis lesions and normal endometrium by tissue microdissection. The extracted proteins were examined using a LC‐MS/MS system and the expression profiles of each region were compared. Two hundred and sixty proteins were detected, among which 73 were expressed more in adenomyosis than in normal endometrium. Among these proteins, we focused on overexpression of moesin in adenomyosis. Expression of moesin estimated semiquantitatively using an immunohistochemistry score was higher in adenomyosis than in normal endometrium. In particular, moesin was significanly overexpressed in stromal cells of adenomyosis than in those of normal endometrium. Relative to normal endometrium, moesin was also overexpressed at the RNA level in 9 of 14 cases of adenomyosis and at the protein level in all 14 cases. We also detected activated (phosphorylated) moesin in adenomyosis lesions. The present findings suggest that moesin is characteristically overexpressed and activated in adenomyosis, and that moesin activation may be related to extension of adenomyosis in the myometrium.