DEFENSIN-6 MESSENGER-RNA IN HUMAN PANETH CELLS - IMPLICATIONS FOR ANTIMICROBIAL PEPTIDES IN HOST DEFENSE OF THE HUMAN BOWEL
DEFENSIN-6 MESSENGER-RNA IN HUMAN PANETH CELLS - IMPLICATIONS FOR ANTIMICROBIAL PEPTIDES IN HOST DEFENSE OF THE HUMAN BOWEL
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DOI：
10.1016/0014-5793(93)81160-2
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发表时间：
1993-01-04
期刊：
影响因子：
3.5
通讯作者：
BEVINS, CL
中科院分区：
文献类型：
--
作者：
JONES, DE;BEVINS, CL
人小肠的上皮表面是宿主和管腔微生物环境之间的屏障。一个人类小肠cDNA克隆被发现编码一个新的抗微生物肽防御素家族成员，命名为人类防御素-6。通过Northern blot分析和聚合酶链反应分析确定，该mRNA的组织表达对小肠具有特异性。原位杂交表明，人防御素-6 mRNA定位于Lieberkuhn隐窝的Paneth细胞。人类Paneth细胞中丰富的防御蛋白mRNA的发现支持了这些上皮细胞可能在人类肠道宿主防御中发挥关键作用的观点。这些结果也加强了基于肽的宿主防御在哺乳动物粘膜表面普遍存在的假设。
The epithelial surface of the human small intestine is a barrier between the host and the microbial environment of the lumen. A human small intestine cDNA clone was found to encode a new member of the defensin family of antimicrobial peptides, named human defensin-6. Tissue expression of this mRNA is specific for the small intestine as determined by Northern blot analysis and polymerase chain reaction analysis. In situ hybridization demonstrated that human defensin-6 mRNA localizes to Paneth cells in the crypts of Lieberkuhn. The finding of an abundant defensin mRNA in human Paneth cells supports the notion that these epithelial cells may play a key role in host defense of the human bowel. The results also strengthen the hypothesis that peptide-based host defenses are prevalent at mucosal surfaces in mammals.