Cloning, characterization and expression analysis of a novel CXC chemokine from turbot (Scophthalmus maximus).
Cloning, characterization and expression analysis of a novel CXC chemokine from turbot (Scophthalmus maximus).
复制标题
DOI：
10.1016/j.fsi.2007.01.008
复制
发表时间：
2007-10
影响因子：
4.7
通讯作者：
Yang Liu;Song‐lin Chen;L. Meng;Yuxi Zhang
中科院分区：
文献类型：
--
作者：
Yang Liu;Song‐lin Chen;L. Meng;Yuxi Zhang
趋化因子是趋化性细胞因子的一个超家族，在白细胞趋化性中起重要作用。本文报道了从大菱鲆脾脏cDNA文库中筛选到的一种新的大菱鲆CXC趋化因子。大菱鲆CXC趋化因子cDNA全长包含81 bp的5′非编码区、414 bp的开放阅读框（ORF）和449 bp的3′非编码区，编码137个氨基酸。大菱鲆CXC趋化因子基因有4个外显子和3个内含子。系统发育分析表明，大菱鲆CXC趋化因子聚类除了所有其他CXC趋化因子。RT-PCR结果表明，大菱鲆CXC趋化因子在脾脏和头肾中有高表达。在受精后胚胎发育的早期阶段，大菱鲆CXC趋化因子的低表达水平首先出现在体节阶段。有趣的是，用鳗弧菌攻击后，大菱鲆的肝脏、脾脏和头肾中快速而快速地（5小时）诱导大菱鲆趋化因子。此外，CXC趋化因子的表达也显着增加大菱鲆胚胎细胞（TECs）的攻击后。这些结果表明，大菱鲆CXC趋化因子在大菱鲆免疫应答中起重要作用。
Chemokines represent a superfamily of chemotactic cytokines playing an important role in leucocyte chemotaxis. Here we report a novel turbot CXC chemokine screened from a turbot spleen cDNA library. The complete cDNA of the turbot CXC chemokine contains an 81bp 5′ UTR, a 414bp open reading frame (ORF) encoding 137 amino acids and a 449bp 3′ UTR. Four exons and three introns are identified in the turbot CXC chemokine gene. Phylogenetic analysis showed that the turbot CXC chemokine clustered apart from all other CXC chemokines. RT-PCR demonstrated that turbot CXC chemokine was expressed highly in spleen and head kidney. During the early stages of embryo development after fertilization, it appears that low expression level of turbot CXC chemokine was firstly observed at somites stage. Interestingly, the turbot chemokine was highly and rapidly (5h) induced in liver, spleen and head kidney of turbot after challenge with Vibrio anguillarum. Furthermore, the expression of CXC chemokine was also dramatically increased after challenge in turbot embryonic cells (TECs). These results indicated that the turbot CXC chemokine played an important role in turbot immune response.