Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis
Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis
复制标题
DOI：
10.1111/j.1600-0625.2011.01306.x
复制
发表时间：
2011-09-01
影响因子：
3.6
通讯作者：
Iwasaki, Toshiroh
中科院分区：
文献类型：
--
作者：
Yoon, Ji-Seon;Nishifuji, Koji;Iwasaki, Toshiroh
角质层（SC）中的神经酰胺（CERs）被认为在皮肤屏障功能中起关键作用。据报道，人类SC含有11种游离的CER类，它们的谱在患有特应性皮炎（AD）的人类中发生改变。尽管在患有AD的狗中有报道称SC中游离cer的比例或蛋白结合cer的数量减少，但犬SC中cer的总体情况尚未完全阐明。因此，本研究的目的是研究犬SC中游离CER的特征，并确定AD犬中CER特征的变化。正相液相色谱电喷雾电离质谱分析显示，游离CER有11个簇，与人类SC中识别的簇相似。采用高效薄层色谱法定量比较了AD犬与品种和年龄匹配的健康犬的游离SC CER。已知在阿尔茨海默病患者皮肤中降低的CER[EOS]、CER[EOP]和CER[NP]在阿尔茨海默病患者狗的皮肤中也降低。这些发现突出表明犬AD是一种自发的动物模型，可用于研究相应人类疾病中与cer相关的皮肤屏障功能的破坏。
Ceramides (CERs) in the stratum corneum (SC) are thought to play a key role in cutaneous barrier function. It has been reported that human SC contains 11 free CER classes and that their profiles are altered in humans with atopic dermatitis (AD). Although decreased proportions of free CERs or quantities of protein-bound CERs in the SC have been reported in dogs with AD, the overall profile of CERs in the canine SC has not been fully elucidated. The aim of this study was thus to investigate the profile of free CERs in the canine SC and to identify alterations in the CER profiles in dogs with AD. Normal-phase liquid chromatography electrospray ionization mass spectrometry indicated 11 clusters of peaks for free CER classes, similar to those recognized in the human SC. The fractions of free SC CER in dogs with AD and in breed- and age-matched healthy dogs were quantitatively compared using high-performance thin-layer chromatography. CER[EOS], CER[EOP] and CER[NP], which are known to be decreased in the skin of humans with AD, were also decreased in the skin of dogs with AD. These findings highlight canine AD as a spontaneous animal model for investigating the disruption of CER-associated cutaneous barrier functions in the corresponding human disease.