Transport and Polymerization of Porphyromonas gingivalis Type V Pili
Transport and Polymerization of Porphyromonas gingivalis Type V Pili
复制标题
V 型菌毛牙龈卟啉单胞菌的转运和聚合
DOI：
10.1007/978-1-0716-0939-2_7
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Nakayama K.
中科院分区：
文献类型：
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作者：
Shoji M;Shibata S;Naito M;Nakayama K.
细菌中的黏附菌毛（或菌毛）分为五种类型，其中V型菌毛最近被描述。V型毛在运输机制、结构和毛素合成方面与其他类型的毛不同。V型菌毛的基因局限于拟杆菌门。组成V型菌毛的蛋白质亚基作为脂蛋白前体被运输到细胞表面，然后通过链交换机制聚合成菌毛，包括棕榈酸标记和Cys-Cys交联分析在内的几个实验证明了这一点。在这里，我们描述了使用这些方法来分析V型菌毛。
Adhesive pili (or fimbriae) in bacteria are classified into five types, among which type V pili have been most recently described. Type V pili differ from other pili types with respect to transport mechanism, structure, and pilin synthesis. Genes of type V pili are restricted to the phylum Bacteroidetes. Protein subunits that compose type V pili are transported to the cell surface as lipoprotein precursors and then polymerized into a pilus through a strand-exchange mechanism, which is demonstrated by several experiments, including palmitic acid labeling and Cys-Cys cross-linking analysis. Here, we describe the use of these methods to analyze type V pili.