Enhancement of anti-tumor effect of particulate vaccine delivery system by 'Bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles
Enhancement of anti-tumor effect of particulate vaccine delivery system by 'Bacteriomimetic' CpG functionalization of poly-lactic-co-glycolic acid nanoparticles
复制标题
DOI：
10.2217/nnm.14.144
复制
发表时间：
2015-01-01
期刊：
影响因子：
5.5
通讯作者：
Jones, Harlan P.
中科院分区：
文献类型：
--
作者：
Kokate, Rutika A.;Thamake, Sanjay I.;Jones, Harlan P.
目的：低免疫原性仍然是实现癌症疫苗全部潜力的主要障碍。在这项研究中，我们评估了CpG包被的肿瘤抗原（Tag）封装的“拟细菌”纳米颗粒（CpG-纳米颗粒[NP]-Tag NPs）作为增强抗肿瘤免疫的方法。材料与方法：合成CpG-NP-Tag NPs，表征其理化性质并进行体内测试。结果如下：我们发现，与相应的对照组相比，CpG预给药后腹膜内（IP）免疫接种CpG-NP-Tag NP显着减弱了雌性BALB/c小鼠的肿瘤生长。组织学和免疫荧光数据显示CpG-NP-Tag肿瘤具有较低的增殖、较高的凋亡活性、较高的CD 4（+）和CD 8（+）T细胞浸润以及较高的IFN γ。与对照组相比。结论：CpG-NP-Tag纳米粒可增强纳米肿瘤疫苗系统的抗肿瘤作用。
Aim: Low immunogenicity remains a major obstacle in realizing the full potential of cancer vaccines. In this study, we evaluated CpG-coated tumor antigen (Tag)encapsulating 'bacteriomimetic' nanoparticles (CpG-nanoparticle [NP]-Tag NPs) as an approach to enhance anti-tumor immunity. Materials & methods: CpG-NP-Tag NPs were synthesized, characterized for their physicochemical properties and tested in vivo. Results: We found CpG predosing followed by intraperitoneal (IP) immunization with CpG-NP-Tag NPs significantly attenuated tumor growth in female BALB/c mice compared with respective controls. Histopathological and Immunofluorescence data revealed CpG-NP-Tag tumors had lower proliferation, higher apoptotic activity, greater CD4(+) and CD8(+) T cell infiltration as well as higher IFN gamma. levels as compared with control groups. Conclusion: Our findings suggest CpG-NP-Tag NPs can enhance anti-tumor effect of nanoparticulate tumor vaccination system.