Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili.
Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili.
复制标题
四型皮利的表达影响了奈瑟氏球菌对VI型分泌系统的杀伤作用。
DOI：
10.7554/elife.63755
复制
发表时间：
2021-07-07
期刊：
影响因子：
7.7
通讯作者：
Exley RM
中科院分区：
文献类型：
--
作者：
Custodio R;Ford RM;Ellison CJ;Liu G;Mickute G;Tang CM;Exley RM
VI型分泌系统（T6SS）广泛存在于细菌中，可以通过介导接触依赖性杀伤来决定多微生物生态系统的发育和组织。在奈瑟氏菌属物种中，包括人类呼吸道的灰奈瑟氏菌，细菌间的接触是由四型皮利（Tfp）介导的，其促进聚集体的形成并支配生长的奈瑟氏菌小菌落的空间动力学。在这里，我们表明，N。灰霉病表达质粒编码的T6SS，其是活性的并且可以限制相关病原体的生长。探讨了Tfp对N.灰霉病T6SS依赖性杀死在一个殖民地，并显示菌毛表达的猎物株增强易感性T6SS相比，非piliated猎物，通过防止隔离T6SS挥舞攻击。我们的研究结果对于理解接触依赖性拮抗作用期间的空间限制如何塑造微生物群落的进化具有重要意义。
Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In Neisseria species, including Neisseria cinerea a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing Neisseria microcolonies. Here, we show that N. cinerea expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on N. cinerea T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities.