Gap junctions and fluid flow response in MC3T3-E1 cells
Gap junctions and fluid flow response in MC3T3-E1 cells
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DOI：
10.1152/ajpcell.2001.281.6.c1917
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发表时间：
2001-12-01
影响因子：
5.5
通讯作者：
Jacobs, CR
中科院分区：
文献类型：
--
作者：
Saunders, MM;You, J;Jacobs, CR
在目前的研究中，我们研究了间隙连接在振荡流体流诱导的成骨细胞内Ca 2+浓度和前列腺素释放的变化中的作用。这项工作在具有完整间隙连接通讯的MC 3 T3-E1细胞以及通过表达显性阴性连接蛋白而导致通讯缺陷的MC 3 T3-E1细胞中完成。我们的研究结果表明，具有完整缝隙连接的MC 3 T3-E1细胞对振荡流体流的反应是前列腺素E-2（PGE（2））释放显著增加，而具有减少缝隙连接通讯的细胞则没有。此外，我们发现，胞质Ca 2+（Ca-1（2+））的反应是不变的中断间隙连接通讯，并没有显着不同的细胞系。因此，我们的研究结果表明，缝隙连接有助于PGE（2），但不是Ca-1（2+）的振荡流体流的响应。这些研究结果牵连间隙连接细胞间通讯（GJIC）在骨细胞整体响应振荡流体流，并建议间隙连接和GJIC在骨的mechanodond转导机制中发挥了关键作用。
In the current study, we examined the role of gap junctions in oscillatory fluid flow-induced changes in intracellular Ca2+ concentration and prostaglandin release in osteoblastic cells. This work was completed in MC3T3-E1 cells with intact gap junctional communication as well as in MC3T3-E1 cells rendered communication deficient through expression of a dominant-negative connexin. Our results demonstrate that MC3T3-E1 cells with intact gap junctions respond to oscillatory fluid flow with significant increases in prostaglandin E-2 (PGE(2)) release, whereas cells with diminished gap junctional communication do not. Furthermore, we found that cytosolic Ca2+ (Ca-i(2+)) response was unaltered by the disruption in gap junctional communication and was not significantly different among the cell lines. Thus our results suggest that gap junctions contribute to the PGE(2) but not to the Ca-i(2+) response to oscillatory fluid flow. These findings implicate gap junctional intercellular communication (GJIC) in bone cell ensemble responsiveness to oscillatory fluid flow and suggest that gap junctions and GJIC play a pivotal role in mechanotransduction mechanisms in bone.