Cloning of Pinus sylvestris SCARECROW gene and its expression pattern in the pine root system, mycorrhiza and NPA-treated short roots
Cloning of Pinus sylvestris SCARECROW gene and its expression pattern in the pine root system, mycorrhiza and NPA-treated short roots
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DOI：
10.1111/j.1469-8137.2007.02102.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Raudaskoski, Marjatta
中科院分区：
文献类型：
--
作者：
Laajanen, Kaisa;Vuorinen, Irmeli;Raudaskoski, Marjatta
SCARECROW（SCR）基因是根辐射状形成的核心基因。它的表达还没有被调查的针叶树形态不同的根类型。本研究克隆了樟子松SCR基因（PsySCR），并通过北方杂交和原位杂交研究了其在樟子松初生根、侧根和短根中的表达。生长素转运抑制剂（NPA）诱导短根二分化。PsySCR在C-末端具有保守的GRAS家族蛋白基序，在N-末端具有可变的GRAS家族蛋白基序。PsySCR在幼根组织和菌根中表达。在根节PsySCR信号贯穿在中柱和根冠柱的首字母的提示，并成为向上限制内皮层在所有根types. PsySCR表达模式表明，第一次的SCR在维持内皮层的特性和径向图案的开放分生组织的根的调节作用。特异性PsySCR定位也是研究短根中二分化过程的极好标记。
The SCARECROW (SCR) gene is central to root radial patterning. Its expression has not been investigated in conifers with morphologically different root types. Additional interest in SCR functions in the Pinus sylvestris root system comes from the effect of ectomycorrhiza formation on the short root apical structure.Here, the P. sylvestris SCR gene (PsySCR) was cloned and its expression investigated by northern blot and in situ hybridization of primary, lateral and short roots and mycorrhiza. Short root dichotomization was induced by auxin transport inhibitor (N-1-naphthylphthalamic acid (NPA)).PsySCR has conserved GRAS family protein motifs at the C-terminus and a variable N-terminus. PsySCR expression occurred in young root tissue and mycorrhiza. In root sections the PsySCR signal runs through the tip in initials for stele and root cap column and becomes upwards-restricted to endodermis in all root types.The PsySCR expression pattern suggests for the first time a regulatory role for SCR in maintaining the endodermal characteristics and radial patterning of roots with open meristem organization. The specific PsySCR localization is also an excellent marker for investigation of the dichotomization process in short roots.