THE 1ST STEP IN VISION - FEMTOSECOND ISOMERIZATION OF RHODOPSIN
THE 1ST STEP IN VISION - FEMTOSECOND ISOMERIZATION OF RHODOPSIN
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DOI：
10.1126/science.1925597
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发表时间：
1991-10-18
期刊：
影响因子：
56.9
通讯作者：
SHANK, CV
中科院分区：
文献类型：
--
作者：
SCHOENLEIN, RW;PETEANU, LA;SHANK, CV
使用飞秒光学测量技术已经解决了视觉中的主要事件的动力学。视紫红质的11-顺式视网膜辅基在500纳米处用35飞秒泵浦脉冲激发，并且在450和580纳米之间用10飞秒探测脉冲测量吸收的瞬态变化。在200飞秒内，在540和580纳米之间观察到增加的吸收，表明在这个时间尺度上形成了光产物。这些测量表明，视觉的第一步，视紫红质生色团的11-顺式-> 11-反式扭转异构化，基本上在200飞秒内完成。
The kinetics of the primary event in vision have been resolved with the use of femtosecond optical measurement techniques. The 11-cis retinal prosthetic group of rhodopsin is excited with a 35-femtosecond pump pulse at 500 nanometers, and the transient changes in absorption are measured between 450 and 580 nanometers with a 10-femtosecond probe pulse. Within 200 femtoseconds, an increased absorption is observed between 540 and 580 nanometers, indicating the formation of photoproduct on this time scale. These measurements demonstrate that the first step in vision, the 11-cis --> 11-trans torsional isomerization of the rhodopsin chromophore, is essentially complete in only 200 femtoseconds.