Placental membrane fatty acid-binding protein preferentially binds arachidonic and docosahexaenoic acids
Placental membrane fatty acid-binding protein preferentially binds arachidonic and docosahexaenoic acids
复制标题
DOI：
10.1016/s0024-3205(98)00267-7
复制
发表时间：
1998-06-19
期刊：
影响因子：
6.1
通讯作者：
Dutta-Roy, AK
中科院分区：
文献类型：
--
作者：
Campbell, FM;Gordon, MJ;Dutta-Roy, AK
为了进一步阐明胎盘膜脂肪酸结合蛋白（p-FABP（pm））在母体血浆长链多不饱和脂肪酸（LCPUFA）优先转移通过人胎盘中的作用，研究了纯化的蛋白与各种放射性标记的脂肪酸（二十二碳六烯酸、花生四烯酸、亚油酸和油酸）的直接结合。这些脂肪酸的蛋白质的结合表明，p-FABP（下午）有较高的亲和力和花生四烯酸和二十二碳六烯酸的结合能力相比，亚油酸和油酸。油酸、亚油酸、花生四烯酸和二十二碳六烯酸的表观结合容量（B-max）值为2.0 +/- 0.14、2.1 +/- 0.17、3.5 +/- 0.11、4.0 +/- 0.10 mol/mol p-FABP（pm），而表观解离常数（K-d）值为1.0 +/-0.7、0.73 +/- 0.04、0.45 ± 0.03和0.4 ± 0.02 μ M（n=3）。在人血清白蛋白的情况下，所有脂肪酸的Kd和B-max值分别约为1 μ M和5 mol/mol蛋白质。这些数据提供了直接的证据，p-FABP（下午）的作用，优先螯合母体花生四烯酸和二十二碳六烯酸的胎盘运输到胎儿凭借其优先结合这些脂肪酸。
To elucidate further the role of placental membrane fatty acid-binding protein (p-FABP(pm)) in preferential transfer of maternal plasma long chain polyunsaturated fatty acids (LCPUFA) across the human placenta, direct binding of the purified protein with various radiolabelled fatty acids (docosahexaenoic, arachidonic, linoleic and oleic acids) was investigated. Binding of these fatty acids to the protein revealed that p-FABP(pm) had higher affinities and binding capacities for arachidonic and docosahexaenoic acids compared with linoleic and oleic acids. The apparent binding capacities (B-max) values for oleic, linoleic, arachidonic and docosahexaenoic acids were 2.0 +/- 0.14, 2.1 +/- 0.17, 3.5 +/- 0.11, 4.0 +/- 0.10 mol per mol of p-FABP(pm) whereas the apparent dissociation constant (K-d) values were 1.0 +/- 07, 0.73 +/- 0.04, 0.45 +/- 0.03 and 0.4 +/- 0.02 mu M, respectively (n=3). In the case of human serum albumin, the K-d and B-max values for all fatty acids were around 1 mu M and 5 mol/mol of protein, respectively. These data provide direct evidence for the role of p-FABP(pm) in preferential sequestration of maternal arachidonic and docosahexaenoic acids by the placenta for transport to the fetus by virtue of its preferential binding of these fatty acids.