The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis
The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis
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DOI：
10.4103/0255-0857.43575
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发表时间：
2008-10-01
影响因子：
1.6
通讯作者：
Rathinavel, L.
中科院分区：
文献类型：
--
作者：
Sekar, B.;Selvaraj, L.;Rathinavel, L.
将基于 IS6110 序列的聚合酶链反应 (PCR) 与传统细菌学技术在肺外结核 (EPTB) 实验室诊断中进行比较。纳入 191 份非重复 EPTB 临床样本和 17 份非结核病例样本作为对照。所有样品均经过抗酸杆菌 (AFB) 齐尔-尼尔森染色处理，143 个样品经过结核分枝杆菌培养处理。使用针对结核分枝杆菌复合体插入元件 IS6110 的 123 bp 片段的引物对所有样品进行 PCR 扩增。在总共处理的 191 个样本中，有 34 个 (18) 的 AFB 涂片结果呈阳性。在处理的 143 个样本中，有 31(22) 个样本的 AFB 培养呈阳性。 51（27）个样本中 AFB 涂片或培养呈阳性。在处理的总共 191 个样本中，有 120 (63) 个 PCR 呈阳性。在 140 个样本中，两种常规技术均呈阴性，而仅通过 PCR 检测，就有 74 (53) 个样本呈阳性。常规技术检测呈阳性的 51 份样本中，PCR 检测呈阳性的有 46 份（90 份）。针对 IS6110 的 PCR 检测可用于建立 EPTB 的诊断，这种诊断存在强烈的临床怀疑，特别是当传统技术呈阴性时。
IS6110 sequence based polymerase chain reaction (PCR) was compared with conventional bacteriological techniques in the laboratory diagnosis of extra-pulmonary tuberculosis (EPTB). One hundred and ninety one non-repeated clinical samples of EPTB and 17 samples from non-tuberculous cases as controls were included. All the samples were processed for Ziehl-Neelsen staining for acid fast bacilli (AFB) and 143 samples were processed by culture for M. tuberculosis . All the samples were processed for PCR amplification with primers targeting 123 bp fragment of insertion element IS6110 of M. tuberculosis complex. Of the total 191 samples processed, 34 (18) were positive by smear for AFB. Culture for AFB was positive in 31(22) samples among the 143 samples processed. Either smear or culture for AFB was found positive in 51(27) samples. Of the total 191 samples processed 120 (63) were positive by PCR. In 140 samples, wherein both the conventional techniques were found negative, 74 (53) samples were positive by PCR alone. Among 51 samples positive by conventional techniques, 46 (90) were found positive by PCR. PCR assay targeting IS6110 is useful in establishing the diagnosis of EPTB, where there is strong clinical suspicion, especially when the conventional techniques are negative.